濒危植物盘龙参种子的非共生萌发及种苗的快繁研究

Asymbiotic seed germination and high-frequency seedling regeneration of Spiranthes sinensis, an endangered orchid

关键词:  盘龙参  非共生萌发  离体快繁

Key words:  Spiranthes sinensis  asymbiotic germination  in vitro high-frequency regeneration

摘要:

以盘龙参种子为材料,筛选离体条件下适宜种子非共生萌发、种苗快繁的培养基。结果表明:盘龙参种子在无植物生长物质的培养基中能够萌发,但不能发育成苗; 在含有1.0 mg·L^-1 KT、0.1 mg·L^-1 IAA和0.1 mg·L^-1 GA₃的培养基中萌发,并能进一步发育形成种苗; 在较高浓度生长素(1.2 mg·L^-1 NAA)的培养基中不能萌发。种苗在较高浓度细胞分裂素和较低浓度生长素配比的培养基中能够增殖,最高增殖系数可达到2.8,转入壮苗生长培养基中培养80 d后可以移栽温室。最适增殖培养基为1/2MS+12.0 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+10.0 mg·L^-1腺嘌呤; 最适壮苗生根培养基为1/2MS+1.0 mg·L^-1 KT+0.1 mg·L^-1 IAA+10.0 mg·L^-1腺嘌呤。

Abstract:

Seeds of Spiranthes sinensis were used to find out appropriate media for in vitro germination and seedling regeneration. Results showed that seeds could germinate on medium without plant growth substances,but failed to develop into seedlings; seedlings formed on medium with 1.0 mg·L^-1 KT,0.1 mg·L^-1 IAA and 0.1 mg·L^-1 GA₃; while seeds could not germinate on media containing lower concentration auxin(1.2 mg·L^-1 NAA). Seedlings could remain high proliferation rate(2.8 times)on media containing higher concentrations kinin and lower concentration auxin,they were eventually transplanted to greenhouse after cultured on hardening medium for 80 d. The suitable seedling proliferation and hardening media were 1/2MS+12.0 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+10.0 mg·L^-1 adenine and 1/2MS+1.0 mg·L^-1 KT+0.1 mg·L^-1 IAA+10.0 mg·L^-1 adenine,respectively.