广西五种绞股蓝属植物离体快繁与种质保存研究

Study on rapid propagation and germplasm conservation in vitro of five species of genus Gynostemma in Guangxi

关键词:  绞股蓝  扁果绞股蓝  广西绞股蓝  光叶绞股蓝  长梗绞股蓝  组织培养  离体保存

Key words:  Gynostemma pentaphyllum  G. guangxiense  G. compressum  G. laxum  G. longipes  tissues culture  conservation in vitro

摘要:

以绞股蓝属植物的带芽茎段为材料,研究不同6-BA浓度与NAA 0.02 mg·L^-1组合对其诱导、分化和增殖的影响,并建立离体快繁体系。结果表明:MS+6-BA 2.0 mg·L^-1+NAA 0.02 mg·L^-1最适宜初代诱导,MS+6-BA 2.0 mg·L^-1+NAA 0.02 mg·L^-1最适合扁果绞股蓝的增殖培养,而MS+6-BA 1.5 mg·L^-1+NAA 0.02 mg·L^-1是其它四种植物增殖的最佳培养基,在1/2MS+NAA 1. 0 mg·L^-1上的生根率均达100%。1/2MS与蔗糖40 g·L^-1对五种植物的保存效果均最好; 添加生长抑制剂能有效减缓生长速度,最佳生长抑制剂为ABA和CCC,浓度均为1.0 mg·L^-1,其中CCC能适合多个物种,连续保存360 d的存活率均在94.5%以上; PP₃₃₃不适合五种植物的保存。活力检测表明,各种质经保存后增殖、生根能力均未下降。

Abstract:

Using stem with buds as explants,experiments were conducted to observe the effects of different concentration 6-BA and NAA 0.02 mg·L^-1on induction,differentiation,proliferation of Gynostemma in Guangxi,in order to establish their rapid propagation systems. Meanwhile,different concentrations of the mineral nutrition,sucrose and plant grow inhibitors(CCC,ABA,PP₃₃₃)were researched. The results showed that the best induction medium was MS medium supplemented with 2.0 mg·L^-16-BA and 0.02 mg·L^-1NAA. The suitable proliferation medium for G. compressum was MS+6-BA 2.0 mg·L^-1+NAA 0.02 mg·L^-1 and MS medium supplemented with 1.5 mg·L^-16-BA and 0.2 mg·L^-1NAA was best for others. The rooting medium was 1/2MS medium with 0.02 mg·L^-1 NAA,accounting for 100% of rooting rate. 1/2MS medium and sucrose 40 g·L^-1 were the most suitable for the conservation in vitro of all species. Plant grow inhibitors could slow down the grow speed effectively,and the best kind and concentration were ABA 1.0 mg·L^-1and CCC 1.0 mg·L^-1. Several species were inhibited by CCC 1.0 mg·L^-1and the survival rate was up to 94.5% after being conserved 360 d. PP₃₃₃ was not suitable for all species to conserve in vitro. Vigor testing showed that the ability of propagating and rooting of the plantlets didn't fall down.