木榄胚轴中一个新生物碱及其抗乙肝病毒活性研究

A new antiviral alkaloid from the hypocotyl of Bruguiera gymnorrhiza

关键词:  天然产物研究，生物碱，乙肝病毒表面抗原，乙型肝炎e抗原，治疗指数

Key words:  natural product research,alkaloid,HbsAg,HbeAg,therapeutic index

摘要:

木榄为红树科木榄属植物，其在广西沿海地区有用作食物的传统，同时有消炎、治疟疾和清热解毒等功效。该研究以采自广西北仑河口国家级自然保护区的红树木榄（Bruguiera gymnorrhiza）胚轴为对象，应用现代色谱方法（硅胶柱色谱、制备液相及制备薄层色谱等），从中分离获得1个生物碱类单体化合物。运用光谱分析技术(HRMS、1H NMR、13C NMR、DEPT、1H-1H COSY、HSQC、HMBC)鉴定其化学结构，发现为新化合物，命名为Gymnorrhizin A。运用MTT法，发现该化合物在对乙肝病毒表面抗原（HbsAg）和乙型肝炎e抗原（HbeAg）的50%抑制浓度（IC50）分别为4.37 mmol•L-1和4.89 mmol•L-1，治疗指数（TI）分别为2.68和2.40。通过对红树木榄胚轴化学成分进行深入研究，阐明其中抗乙肝病毒活性物质基础，为相关新药研发提供了科学依据。

Abstract:

Bruguiera gymnorrhiza is a common buttressed tree found in the mangrove forests. The hypocotyl of a mangrove B. gymnorrhiza are traditionally used as food and herbal medicine in Guangxi. A new alkaloid (1) was isolated from the hypocotyl of a mangrove B. gymnorrhiza and purified by repeated column chromatography on silica and HPLC. The structure of Compound 1 was elucidated on the basis of extensive spectroscopic analysis (HRMS，1H NMR，13C NMR，DEPT，1H-1H COSY，HSQC and HMBC). Compound 1 was a new compound by the method of SciFinder scholar，and it showed that anti-Hepatitis B virus (HBV) activity against HbsAg and HbeAg with IC50 values of 4.37 mmol•L-1 and 4.89 mmol•L-1，and TI values of 2.68 and 2.40，respectively. The hypocotyl of a mangrove B. gymnorrhiza has been commonly used as crude material of herbal medicine for a long time，and this study would ac-cumulate data for chemical constituents research of the genus.