摘要: |
为了探讨气候因子对不同季节授粉罗汉果品质影响及其分子作用机制,该文以主栽“青皮果”品种夏季与秋季授粉果实为研究对象,采用不同发育时间气候因子监测、形态大小测量、罗汉果苷代谢检测和基因表达qRT-PCR分析方法,统计分析二者生境气候因子、品质性状和基因表达差异。结果表明:(1)秋季授粉果实与夏季授粉果实相比,生境平均温度和有效积温35 d以后明显降低,昼夜温差65 d以前明显增加,并且平均温度、有效积温差异大于昼夜温差,光照强度和空气湿度则相近。(2)横径、纵径和单果重均增加,但是差异不显著。(3)罗汉果苷Ⅴ和11-O-罗汉果苷Ⅴ合成积累速度滞后10 d左右,含量从55 d开始均显著降低并且成熟时分别降低了40.66%和46.07%。(4)罗汉果苷Ⅴ合成酶基因55 d协同表达的一致性差,上调数目和幅度少,而且负责最后一步合成反应的葡萄糖基转移酶基因SgUGT94-289-3均下调表达。综上所述,夏季与秋季授粉罗汉果形态大小未受气候因子影响,而罗汉果苷Ⅴ品质则受温度影响显著,温度可能通过调控罗汉果苷Ⅴ合成酶基因协同表达的一致性和水平致使春季和秋季的罗汉果苷Ⅴ品质具有差异性。该研究结果为罗汉果优质栽培和遗传育种提供了理论依据。 |
关键词: 不同季节, 罗汉果品质, 气候因子, 果实大小, 罗汉果苷Ⅴ含量, 基因表达 |
DOI:10.11931/guihaia.gxzw202308077 |
分类号:Q943 |
文章编号:1000-3142(2024)07-1352-12 |
Fund project:国家自然科学基金区域创新发展联合基金重点项目(U20A2004); 湖南省科技厅重点研发项目(2022NK2004); 财政部和农业农村部国家现代农业产业技术体系项目(CARS-21); 广西农业科学院基本科研业务专项(桂农科2024YP091)。 |
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Effects of climatic factors on fruit quality of Siraitia grosvenorii and its molecular regulation mechanism |
PENG Huan1, MO Changming2, XIE Lei3, LUO Zuliang3,
GUO Wenfeng2, TANG Qi4, XIAO Dong1*
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1. College of Agriculture, Guangxi University, Nanning 530004, China;2. Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi
Academy of Agricultural Sciences, Nanning 530007, China;3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking
Union Medical College, Beijing 100193, China;4. College of Horticulture, Hunan Agricultural University, Changsha 410128, China
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Abstract: |
In order to investigate the impact of climatic factors on the quality of Siraitia grosvenorii fruits pollinated in different seasons and the underlying molecular mechanism involved, the differences in climate factors, quality traits, and gene expression between fruits of the main cultivar S. grosvenorii “Qingpiguo” pollinated in summer and autumn were statistically analyzed by monitoring climate factors at different developmental stages, measuring morphological changes, detecting mogroside metabolism, and analyzing gene expression using qRT-PCR. The results were as follows:(1)Compared to summer-pollinated fruits, the average temperature and effective accumulated temperature of autumn-pollinated fruits decreased significantly after 35 d. Additionally, the temperature difference between day and night increased significantly before 65 d. However, this difference was still less than that of the average temperature and effective accumulated temperature. The light intensity and air humidity remained similar.(2)The transverse diameters, longitudinal diameters, and single fruit weights of autumn-pollinated fruits increased compared to those of summer-pollinated fruits, however, these differences were not statistically significant.(3)Mogroside V and 11-O-mogroside V in autumn-pollinated fruits accumulated slowly from 55 d with a delay of about 10 d, moreover, the content of both compounds in ripe fruits decreased by 40.66% and 46.07%, respectively.(4)In autumn, the number and extent of up-regulated mogroside V synthetase genes were relatively lower, and their co-expression consistency was poorer than in summer. Furthermore, the glucosyltransferase gene SgUGT94-289-3 which was responsible for the final step in mogroside V biosynthesis, exhibited down-regulation at all time points of 55 d. In summary, the shape and size of S. grosvenorii fruits pollinated in different seasons were not significantly affected by climatic factors; however, the content of mogroside V was significantly influenced by the temperature, which potentially influences these variations in mogroside V by regulating both the co-expression consistency and expression level of mogroside V synthetase genes. The results of this study provide the theoretical reference for high-quality cultivation and genetic breeding of S. grosvenorii. |
Key words: different seasons, Siraitia grosvenorii quality, climatic factors, fruit size, mogroside V content, gene expression |