砂生槐根瘤内生菌对青稞种子萌发及幼苗的促生作用

Effects of Sophora moorcroftiana endophytic bacteria on germination of black highland barley seeds and seedling growth

关键词:  砂生槐, 根瘤内生菌, 黑青稞, 种子萌发, 促生

Key words:  Sophora moorcroftiana, endophytic bacteria in root nodules, black highland barley, seed germination, promote growth

摘要:

该文以接种不同砂生槐根瘤内生菌的黑青稞种子和不接种菌株的黑青稞种子为研究材料,采用培养皿作为基质,观察黑青稞种子萌发及幼苗生长情况,测定接种不同根瘤内生菌后的黑青稞发芽率、发芽势、发芽指数、株高、根长、须根数、根冠比、植株全株干/鲜重的生理性状,并对各不同处理进行隶属函数综合评价。结果表明:菌株R7和R17的发芽率均达到了100%,R17、R18及R19的发芽势达到了90%,R17和R18的发芽指数变化量与CK1对比分别为45.38%和32.05%。在不同处理下,R21、R14与R7的平均根长与CK1对比呈抑制作用,其变化量分别为-50%、-34.28%及-28.99%; 黑青稞幼苗植株高与CK1对比,仅有4株菌对株高产生促进作用,分别是R5、R18、R22及R28; 黑青稞幼苗须根数与CK1对比,除R15与R21呈抑制作用外,其余菌株均对幼苗须根数产生促进作用,但R17、R18及R19处理下须根数无显著差异(P>0.05)。与CK1对比,对植株全株鲜重产生促进作用的仅有4株菌,分别为R2、R18、R22和R28; 菌株R5、R6及R14的全株干重均低于对照CK1,其余与CK1对比差异显著(P<0.05)。综上结果显示,不同砂生槐根瘤内生菌菌株对黑青稞的种子萌发及幼苗生理性状影响差异显著(P<0.05); 接种不同根瘤内生菌效应的综合评价结果显示,接种R17、R18、R28、R20、R19菌株的黑青稞种子隶属函数值为6.71、6.45、6.31、5.81、5.76,可初步选为优良的促生菌株。

Abstract:

In order to study the effects of inoculating different sophora moorcroftiana nodule endophytes on the germination and physiological traits of black highland barley seeds, the endophytic bacteria of sophora moorcroftiana nodules which promoted the growth of black highland barley seedlings were screened. Black highland barley seeds inoculated with endophytic bacteria of different sophora moorcroftiana nodules and black highland barley seeds not inoculated with strains were used as research materials, and culture dishes were used as substrates to observe the seed germination and seedling growth of black highland barleys. The germination rate, germination potential, germination index, plant height, root length, root number, root-shoot ratio, physiological characteristics of plant dry/fresh weight of black highland barley after inoculation with different endophytic bacteria were determined, and the different treatments were subordinated. Comprehensive evaluation of functions. The results showed that the germination rates of strains R7 and R17 reached 100%, the germination potential of R17, R18 and R19 reached 90%, and the germination indexes of R17 and R18 were 45.38% and 32.05%, respectively. Under different treatments, the average root length of R21, R14 and R7 was inhibited by CK1, and the changes were -50%, -34.28% and -28.99%, respectively. The plant height of black highland barley seedlings was compared with CK1, and only four strains promoted plant height, which were R5, R18, R22 and R28. The number of roots of black highland barley seedlings was compared with CK1, Except that R15 and R21 inhibited, the other strains promoted the number of seedlings of seedlings, but there was no significant difference in the number of roots under R17, R18 and R19 treatments(P>0.05). Compared with CK1, there were only four strains that promoted the fresh weight of plants, which were R2, R18, R22 and R28. The dry weight of strains R5, R6 and R14 were lower than that of control CK1, and the others had significant differences compared with CK1(P<0.05). The results indicate that the different endophytic strains of different sophora moorcroftiana nodules have significant effects on the seed germination and seedling physiological traits of black highland barley(P<0.05). The comprehensive evaluation results of inoculating different nodule endophytic effects showed that R17, R18, R28 and R20 were inoculated. The sub-function value of the black highland barley seed of R19 strain is 6.71, 6.45, 6.31, 5.81, 5.76, which can be initially selected as an excellent growth-promoting strain.