| 摘要: |
| 为揭示影响苦荞籽粒黄酮含量的主要因素,获得高黄酮含量的苦荞种质,该文以“小米荞×晋荞2号”重组自交系(RILs)群体为材料,采用酶标仪检测籽粒黄酮含量,调查了百粒重、籽粒长宽比、株高、分枝数及籽粒产量等性状的变异,并探究籽粒黄酮含量与产量性状之间的相关关系,比较了不同粒色、不同粒型苦荞株系的籽粒黄酮含量的差异,基于聚类分析筛选了高黄酮含量和高产优异株系。结果表明:(1)父本‘晋荞2号'的籽粒黄酮含量、籽粒产量、百粒重和籽粒长宽比均极显著高于母本‘小米荞'; RILs群体中籽粒黄酮含量的范围是1.34%～3.05%,变异系数为12.23%; 产量性状的变异系数范围在9.38%～25.17%,其中籽粒产量最大,百粒重次之,籽粒长宽比最小; 籽粒黄酮含量和产量性状呈现连续地近似正态分布,并存在明显的双向超亲现象。(2)苦荞籽粒黄酮含量与产量性状均不存在显著相关性; 籽粒产量与百粒重、株高存在极显著正相关。(3)不同粒色和不同果壳类型的株系间籽粒黄酮含量差异不显著。(4)在欧式距离为17.6～20.6处,219个株系可聚为6类,其中C3类群和C6类群籽粒黄酮含量最高,均值分别为2.39%和2.35%,C2类群在籽粒产量、株高、分枝数、百粒重、籽粒长宽比等方面表现良好。 |
| 关键词: 苦荞, 重组自交系, 黄酮含量, 产量性状, 聚类分析 |
| DOI：10.11931/guihaia.gxzw201906046 |
| 分类号:Q945 |
| 文章编号:1000-3142(2021)02-0216-09 |
| Fund project:国家自然科学基金(31460280,31660424); 贵州省科技厅科学技术基金(黔科合JZ字 [2014]2011号,黔科合LH字 [2016]7224号,黔科合基础 [2016]1106号); 贵州省荞麦种质资源保育及创新重点实验室建设基金(黔教合KY字 [2017]002)[Supported by the National Natural Science and Technology Foundation of China(31460280, 31660424); Natural Science and Technology Foundation of Guizhou Provincial Department of Science and Technlology([2014]2011, [2016]7224, [2016]1106); Earmarked Fund for construction of the Key Laboratory for Conservation and Innovation of Buckwheat Germplasm in Guizhou([2017]002)。 |
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| Variation analysis of flavonoid contents in seeds and yield traits on recombinant inbred line population of tartary buckwheat |
|
ZHENG Ran1,2, LI Ruiyuan3, LÜ Dan1,2, ZHENG Junqing1,2,
SHI Taoxiong1*, CHEN Qijiao1, CHEN Qingfu1
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1. Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang 550001, China;2. School of Life Sciences,
Guizhou Normal University, Guiyang 550001, China;3. Key Laboratory of Information and Computing Science of
Guizhou Province, Guizhou Normal University, Guiyang 550001, China
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| Abstract: |
| In order to reveal the main factors affecting the flavonoids content of tartary buckwheat in seeds and select high flavonoids content lines, we determined the flavonoids content in seeds of a tartary buckwheat recombinant inbred lines(RILs)population derived from a cross of “Xiaomiqiao × Jinqiao 2”. The genetic variation of seed yield, 100-seed weight, seed length to width ratio, plant height, branch number and flavonoids content in seeds were analyzed on RILs population. The relationship between flavonoids content in grains and yield traits was explored by correlation analysis. Variance analysis of flavonoids content in RILs population with different seed colors or shell types was carried out, cluster analysis for the RILs population was carried out based on the phenotypic data of investigated traits, and the excellent lines were selected by variance analysis among groups. The results were as follows:(1)The flavonoids content in seeds, 100-seed weight, seed yield, and seed length to width ratio of ‘Jinqiao 2' were significantly higher than those of ‘Xiaomiqiao'; The flavonoids content in seeds of the RILs population ranged from 1.34% to 3.05% with the coefficient variation of 12.23%; The coefficient of variation of yield traits ranged from 9.38% to 25.17%, in which grain yield was the largest, followed by 100-seed weight, and seed length to width ratio was the smallest; The tremendous transgressive segregation for flavonoids content in seeds was observed in the population.(2)There was no significant correlation between flavonoids content in seeds and the investigated agronomic traits; Seed yield was significantly positively correlated to 100-seed weight and plant height.(3)There were no significant differences in flavonoids content among lines with different seed colors or shell types.(4)The RILs population could be divided into six groups at the euclidean distance of 17.6-20.6. Group C3 and Group C6 had the highest flavonoids content in seeds, with the average values of 2.39% and 2.35% respectively. Group C2 had fine performance in plant height, branch number, seed yield, 100-seed weight, and seed length to width ratio. |
| Key words: tartary buckwheat, recombinant inbred line, flavonoids content, yield traits, cluster analysis |
