| 摘要: |
| 全缘凤尾蕨资源丰富且在贵州分布广泛,具有清热利湿、活血消肿的功效。为了探明该植物的物质基础、寻找相关的活性成分和先导化合物,该文用95%甲醇对全缘凤尾蕨地上部分浸渍提取,采用硅胶、MCI gel CHP 20P、YMC gel ODS-A-HG、Sephadex LH-20等色谱技术对全缘凤尾蕨的化学成分进行分离纯化,根据波谱数据鉴定化合物的结构。结果表明:从全缘凤尾蕨中分离得到18个化合物,分别鉴定为(-)-α-tocospirone(1)、环鸦片甾烯醇(2)、(2S,3S)-pterosin C(3)、(2R,3S)-pterosin C(4)、pterosin B(5)、pterosin F(6)、α-紫罗兰酮A(7)、sauropunol C/D(8/8')、ficusol(9)、棕榈酸(10)、2-dodec-2-enyl-succinic acid dimethyl ester(11)、methyl-9-phenyl-10-hydroxyoctadecanoate(12)、十六烷酸甘油酯(13)、methyl elaidate(14)、(Z,Z)-9,12-十八烷二烯酸甲酯(15)、(Z,Z,Z)-9,12,15-十八烷三烯酸甲酯(16)、胡萝卜苷(17)、β-谷甾醇(18)。所有化合物均为首次从该植物中分离得到。该研究结果为合理利用其资源奠定了科学基础。 |
| 关键词: 凤尾蕨属, 全缘凤尾蕨, 化学成分, 分离纯化, 结构鉴定 |
| DOI：10.11931/guihaia.gxzw202001035 |
| 分类号:Q946 |
| 文章编号:1000-3142(2021)07-1046-08 |
| Fund project:国家自然科学基金(81660723); 贵州省科技计划项目 [黔科合平台人才(2017)5618]; 贵州省一流学科项目[GNYL(2017)008号-7-Y]; 贵州中医药大学大学生创新创业训练计划项目 [贵中医大创合字(2017)13号]; 贵州中医药大学药学院卓越计划项目(15号)[Supported by the National Natural Science Foundation of China(81660723); Youth Science and Technology Talent Project of Guizhou Province(2017-5618); Guizhou Province First-Class Disciplines Project(GNYL2017-008-7-Y); Undergraduate Training Program for Innovation and Entrepreneurship of Guizhou University of Traditional Chinese Medicine(2017-13); School of Pharmacy Excellence Program of Guizhou University of Traditional Chinese Medicine(15)]。 |
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| Chemical constituents from Pteris insignis |
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ZOU Juan, MAO Xiaoxian, HE Kang*, YE Jianghai, TANG Rentao,
WANG Yuxue, ZHANG Jingjie
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Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
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| Abstract: |
| Pteris insignis, which has the medical function of clearing heat and draining dampness as well as activating blood to subside swelling, is widely distributed and rich in resources in Guizhou. As far as we know, there is no literature report on the study of its chemical constituents and biological activity. The purpose of this paper was to explore the material basis of this species, to reveal the related bioactive constituents and lead compounds. The aerial part of P. insignis was extracted with 95% methanol to give crude extract, which was then isolated and purified by silica gel, MCI gel CHP 20P, YMC gel ODS-A-HG and Sephadex LH-20 column chromatography. The structures of obtained compounds were deduced by the analysis of comprehensive spectral data. Eighteen compounds were isolated and identified as(-)-α-tocospirone(1), cyclolaudenol(2),(2S,3S)-pterosin C(3),(2R,3S)-pterosin C(4), pterosin B(5), pterosin F(6), α-ionone A(7), sauropunol C/D(8/8'), ficusol(9), palmitic acid(10), 2-dodec-2-enyl-succinic acid dimethyl ester(11), methyl-9-phenyl-10-hydroxyoctadecanoate(12), hexadecanoic acid-2,3-dihydroxy-propyl ester(13), methyl elaidate(14),(Z,Z)-9,12-octadecadienoic acid methyl ester(15),(Z,Z,Z)-9,12,15-octadecatrienoic acid ester(16), carotenoid(17), β-sitosterol(18). All the compounds were isolated from P. insignis for the first time. The results of this study will lay a scientific foundation for the rational use of its resources. |
| Key words: Pteris L., Pteris insignis, chemical constituents, isolation and purification, structure identification |
