| 摘要: |
| 山豆根为豆科植物越南槐(Sophora tonkinensis)的干燥根和根茎,常用于治疗咽喉部感染性疾病,其大极性提取物已经报道了具有较好的抑菌活性潜力。为了深入探究山豆根大极性抗菌活性成分,该文综合运用硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱、半制备HPLC等分离纯化方法对山豆根正丁醇部位化学成分进行分离鉴定,并开展了单体成分抗细菌(粪肠球菌、金黄色葡萄球菌、绿脓杆菌、大肠杆菌)和真菌(白色念珠菌)的活性评价。结果表明:从山豆根正丁醇部位中共分离鉴定得到10 个化合物,分别为2',4',7-trihydroxy-6,8-bis(3-methyl-2-butenyl)flavanone(1)、染料木苷(2)、2-(2',4'-二羟基苯基)-5,6-二氧亚甲基苯并呋喃(3)、maltol-β-D-glucopyranoside(4)、leonuriside A(5)、松柏醇-9-O-β-D-吡喃葡萄糖苷(6)、光甘草酚(7)、2-(2',4'-dihydroxy-phenyl)-8,8-dimethyl-10-(3-methyl-2-butenyl)-8H-pyrano [2,3-d]chroman-4-one(8)、氧化苦参碱(9)、番石榴酸(10),其中化合物4-6首次从该植物中分离得到。生物活性测试结果表明化合物1-3具有一定的抑菌作用,化合物2对绿脓杆菌的最小抑菌浓度(MIC)值为15.6 μg·mL-1,化合物3对白色念珠菌和大肠杆菌的MIC值均为31.3 μg·mL-1。 |
| 关键词: 山豆根, 化学成分, 提取分离, 抑菌活性 |
| DOI：10.11931/guihaia.gxzw202101025 |
| 分类号:Q946 |
| 文章编号:1000-3142(2021)07-1054-07 |
| Fund project:国家重点研发计划(2018YFC1708100); 云南省教育厅科研基金项目(2020J0139); 国家自然科学基金(31900290)[Supported by the National Key Research and Development Program of China(2018YFC1708100); Yunnan Province Education Department Scientific Research Fund Project(2020J0139); the National Natural Science Foundation of China(31900290)]。 |
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| Chemical constituents from the n-butanol extract of Sophora tonkinensis and their antibacterial activities |
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WEI Xin1, ZHANG Wei1, DING Caifeng 2, YU Haofei 2, ZHANG Liyan1, ZHOU Ying1*
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1. Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China;2. Kunming Medical University, Kunming 650500, China
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| Abstract: |
| The dried roots of Sophora tonkinensis were commonly used as the treatment of the throat and other infectious diseases. In order to identify its large polar antibacterial active components, silica gel column chromatography(CC), ODS CC and Sephadex LH-20 CC and semi preparative HPLC were used to isolate the chemical constituents from n-butanol fraction of S. tonkinensis. The antibacterial activities of the monomers against bacteria(Enterobacter faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli)and fungi(Candida albicans)were evaluated. The results showed ten compounds were isolated and identified as 2',4',7-trihydroxy-6,8-bis(3-methyl-2-butenyl)flavanone(1),genistin(2), 2-(2',4'-dihydroxy)-5,6-dioxomethylbenzofuran(3), maltol-β-D-glucopyranoside(4), leonuriside A(5),coniferylalcohol-9-O-β-D-glucopyranoside(6),glabrol(7),2-(2',4'-dihydroxy-phenyl)-8,8-dimethyl-10-(3-methyl-2-butenyl)-8H-pyrano [2,3-d]chroman-4-one(8), oxymatrine(9), piscidic acid(10). Among of them, compounds 4-6 were isolated from the title plant for the first time, for our knowledge. Besides, biological activity test showed the potential antibacterial activities of compounds 1-3. It is worth mentioning that the minimum inhibitory concentration(MIC)value of compound 2 against Pseudomonas aeruginosa was 15.6 μg·mL-1, while the MIC of compound 3 against Candida albicans and Escherichia coli were 31.3 μg·mL-1. |
| Key words: Sophora tonkinensis, chemical constituents, extraction and isolation, antibacterial activities |
