庐山石韦的化学成分研究

Chemical constituents from Pyrrosia sheareri

关键词:  庐山石韦, 民族药, 化学成分, 分离纯化, 结构鉴定

Key words:  Pyrrosia sheareri, ethnic medicine, chemical constituents, isolation and purification, structure identification

摘要:

庐山石韦(Pyrrosia sheareri)为贵州少数民族常用药材,但其化学成分研究较少。为更深入研究其化学成分,该文采用大孔吸附树脂、硅胶色谱、葡聚糖凝胶LH-20色谱等分离纯化技术对其乙醇提取物进行分离纯化,采用波谱数据分析及 参考文献比对等方法对分离得到的化合物进行结构鉴定。结果表明:从庐山石韦全草中分离得到11个化合物,分别鉴定为环阿尔廷-25-烯-3β,24-二醇(1)、3β-羟基齐墩果-12-烯-27-羧酸(2)、乌索酸(3)、7β-羟基谷甾醇(4)、7α-羟基谷甾醇(5)、β-谷甾醇(6)、3,7-二羟基-5-辛内酯(7)、5,7,3',5'-三羟基二氢黄酮(8)、香草酸(9)、咖啡酸甲酯(10)、8-羟基辛酸(11)。其中,化合物1和2均为首次从石韦属植物中分离得到,化合物1、2、4、5、7、8、10、11均为首次从庐山石韦中分离得到。

Abstract:

Pyrrosia sheareri is widely used as folk herbs in minority areas of Guizhou Province. However, its chemical constituents are rarely reported. To further study the chemical constituents of P. sheareri, macroporous adsorption resins, silica-gel and sephadex LH-20 columns were employed to isolate and purify chemical constituents of P. sheareri. The structures of obtained compounds were identified by comprehensive spectroscopic analyses and confirmed by comparison of their spectral data with those in the literature. The results were as follows: Eleven compounds were isolated and elucidated respectively as cycloart-25-en-3β,24-diol(1), 3β-acetoxyolean-12-en-27-oic acid(2), ursolic acid(3), 7β-hydroxysitosterol(4), 7α-hydroxysitosterol(5), β-sitosterol(6), 3,7-dihydroxy-5-octanolide(7), 5,7,3',5'-tetrahydroxy-flavanone(8), vanillic acid(9), caffeic acid methyl ester(10), 8-hydroxyoctanoic acid(11). Compounds 1, 2, 4, 5, 7, 8, 10 and 11 were isolated from this plant for the first time, and compounds 1 and 2 were isolated from this genus for the first time.