尖尾枫化学成分及其抗炎活性研究

Chemical constituents and their anti-inflammatory activities of Callicarpa longissima

关键词:  尖尾枫, 化学成分, 分离鉴定, 抗炎活性

Key words:  Callicarpa longissima, chemical constituents, isolation and identification, anti-inflammatory activity

摘要:

尖尾枫(Callicarpa longissima)有止血镇痛的功效,其化学成分和药理活性研究报道较少。为研究尖尾枫枝叶的化学成分以及抗炎活性,该实验用尖尾枫枝叶95%乙醇提取物通过柱层析和HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用LPS离体诱导RAW 264.7细胞的NO生成模型,研究主要化合物对NO生成的抑制活性。结果表明:(1)从尖尾枫中分离得到12个化合物,分别鉴定为4',5-二羟基-7-甲氧基黄酮(1)、喷杜素(2)、蓟黄素(3)、洋艾素(4)、4',5-二羟基-3',7-二甲氧基黄酮(5)、甲基条叶蓟素(6)、金腰素(7)、泡桐素(8)、齐墩果酸(9)、桦木酸(10)、2,4,6-三甲氧基苯酚(11)、咖啡酸乙酯(12)。(2)化合物1-7在25 μmol·L^-1浓度下对LPS诱导的小鼠巨噬细胞RAW 264.7释放的NO均具有明显的抑制作用。化合物1、2、4、5、6、8、10、11、12均为首次从该植物中分离得到,化合物1-7均具有不同程度的抗炎作用,其中化合物2、3、6表现出较强的抗炎活性。

Abstract:

Callicarpa longissima has hemostatic and analgesic effects, and there are few reports on its chemical composition and pharmacological activity. In order to study the chemical composition and anti-inflammatory activity of C. longissima branches and leaves, the 95% ethanol extract of C. longissima branches and leaves were isolated and purified by column chromatography and HPLC, and the structures of the obtained compound were identified based on the physical and chemical properties and spectral data. Using LPS-activated RAW 264.7 cell line models in vitro, main compounds were evaluated for the inhibition against NO production. The results were as follows: Twelve compounds were isolated and identified as 4',5-dihydroxy-7-methoxyflavone(1), penduletin(2), cirsimaritin(3), artemetin(4), 4',5-dihydroxy-3',7-dimethoxyflavone(5), cirsilineol(6), chrysosplenetin(7), paulownin(8), oleanolic acid(9), betulinic acid(10), 2,4,6-trimethoxyphenol(11), ethyl caffeate(12).(2)Compounds 1-7 have significant inhibitory effect on the release of NO from mouse macrophages RAW 264.7 induced by LPS at a concentration of 25 μmol·L^-1. In conclusion, compounds 1, 2, 4, 5, 6, 8, 10, 11 and 12 were isolated from this plant for the first time. Compounds 1-7 have anti-inflammatory effects, among which compounds 2, 3 and 6 show strong anti-inflammatory activity.