茎花山柚叶的化学成分研究

Chemical constituents from the leaves of Champereia manillana var. longistaminea

关键词:  茎花山柚, 提取分离, 纯化, 化学成分, 结构鉴定

Key words:  Champereia manillana var. longistaminea, extraction and separation, purification, chemical constituents, structure identification

摘要:

为深入认识特色植物茎花山柚(Champereia manillana var. longistaminea)的药效物质基础,该研究开展了茎花山柚叶95%乙醇提取物的化学成分研究。结果表明:(1)采用硅胶柱层析(CC)、薄层色谱(TLC)、葡聚糖凝胶柱层析(Sephadex LH-20)、反相硅胶柱层析(RPC₁₈)与高效液相色谱(HPLC)等方法,对茎花山柚叶乙醇浸提物的乙酸乙酯萃取部位进行了分离纯化,得到6个单体化合物。(2)通过核磁共振波谱(NMR)和高分辨质谱(HR-ESI-MS)数据,并结合文献数据对比鉴定了这些化合物的结构,6个化合物分别为蒲公英赛醇(1)、吲哚-3-甲酸(2)、(24R)-环菠萝蜜烷-3β,24,25-三萜(3)、(24R,S)-3β-24,31-环氧-24-甲基环烷(4)、1-O-亚麻酰基-3-O-β-D-吡喃半乳糖基-sn-丙三醇(5)、长烷基链甘油单酯(6)。其中,化合物1-6均为首次从该植物中分离得到。

Abstract:

In order to study the chemical constituents in the leaves of Champereia manillana var. longistaminea, the chemical composition was analyzed by the silica gel column chromatography(CC), thin-layer chromatography(TLC), dextran gel column chromatography(Sephadex LH-20), reversed-phase silica gel(RPC₁₈)column chromatography and high performance liquid chromatography(HPLC). The ethyl acetate extraction part of the ethanol extraction was separated and six monomer compounds were obtained. The structures of these compounds were identified using nuclear magnetic resonance spectroscopy(NMR), high resolution mass spectrometry(HR-ESI-MS)and the data of the literature. Six compounds were elucidated as taraxerol(1), 1H-indole-3-carboxylic acid(2),(24R)-cycloartane-3β, 24, 25-triol(3),(24R, S)-3β-24,31-epoxy-24-methylcycloartane(4), 1-O-linolenoyl-3-O-β-D-galactospyranosyl-snglycerol(5), and hyloglyceride(6). All compounds 1-6 were isolated from this plant for the first time.