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10 期 陈娇娇等: 镉积累对艾纳香内生菌群落结构和共发生网络的影响 1 8 9 1
Berry 和 Widder(2014) 阐述的复杂网络能更好应 distribution and chemical forms of cadmium in Blumea
对环境变化观点相一致ꎮ 在本研究中ꎬCd 积累下 balsamifera [J]. Seedꎬ 41(2): 31-39. [陈子涵ꎬ 任建国ꎬ
艾纳香器官叶的模块性远低于茎和根ꎬ说明叶部 庞玉新ꎬ 等ꎬ 2022. 镉胁迫对艾纳香抗性生理、亚细胞镉
内生菌共发生网络模块间联系紧密且不同物种分 分布和镉化学形态的影响 [J]. 种子ꎬ 41(2): 31-39.]
CHENG Cꎬ WANG Rꎬ SUN LJꎬ et al.ꎬ 2021. Cadmium ̄
布于不同的模块ꎬ有利于形成特定生态位相对稳
resistant and arginine decarboxylase ̄producing endophytic
定的共生内生菌群落以适应 Cd 积累胁迫ꎮ 因此ꎬ
Sphingomonas sp. C40 decreases cadmium accumulation in
在本研究中ꎬCd 污染可能通过诱导艾纳香建立更
host rice (Oryza sativa cliangyou 513) [J]. Chemosphereꎬ
加复杂的内生菌共发生网络和增强物种间的正向
275: 130109.
关联强度来应对 Cd 积累的影响ꎮ 值得注意的是ꎬ CHU CQꎬ FAN MYꎬ SONG CYꎬ et al.ꎬ 2021. Unveiling
本文并未设置 Cd 对艾纳香生长的抑制浓度且研 endophytic bacterial community structures of different rice
究样本量有限ꎬ存在不足之处ꎮ 因此ꎬ在后续的研 cultivars grown in a cadmium ̄contaminated paddy field
究中将进一步改进研究方案ꎬ使其变得更加深入、 [J]. Front Microbiolꎬ 12: 756327.
FUENTES ̄RAMIREZ LEꎬ CABALLERO ̄MELLADO Jꎬ
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4 结论 Acetobacter diazotrophicus is inhibited by high N ̄fertilization
[J]. Fems Microbiol Ecolꎬ 29(2): 117-128.
GAO Tꎬ SHI XYꎬ 2018. Preparation of a synthetic seed for the
(1)MiSeq 测序表明ꎬCd 积累提高了艾纳香各
common reed harboring an endophytic bacterium promoting
器官内生菌 α 多样性ꎬ各器官 α 多样性总体表现 seedling growth under cadmium stress [ J]. Environ Sci
为根>茎>叶ꎮ (2) RDA 分析表明ꎬ艾纳香器官内 Pollut Res Intꎬ 25(9): 8871-8879.
生菌群落组成与植株器官 Cd 含量和根际土壤 Cd HALLMANN Jꎬ QUADT ̄HALLMANN Aꎬ MAHAFFEE WFꎬ et
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量和根际土壤 Cd 含量呈正相关ꎮ (3) 线性判别分 [J]. Can J Microbiolꎬ 43(10): 895-914.
析(LEfSe)表明ꎬ艾纳香根中内生菌差异物种较茎 HARDOIM PRꎬ VAN OVERBEEK LSꎬ ELSAS JDꎬ 2008.
和叶丰富ꎮ 此外ꎬCd 积累显著增加了根、茎和叶中 Properties of bacterial endophytes and their proposed role in
plant growth [J]. Trends Microbiolꎬ 16(10): 463-471.
的分枝杆菌属、类诺卡氏菌属、慢性根瘤菌属、鞘
HE Lꎬ REN YZꎬ ZENG WMꎬ et al.ꎬ 2021. Deciphering the
氨醇单细胞菌属、乳杆菌属、纤维单胞菌属、芽孢
endophytic and rhizospheric microbial communities of a
杆菌属、Mesorhizobium、Rhizobacter 等属的耐 Cd 内
metallophyte Commelina communis in different Cu ̄polluted
生菌数量ꎮ (4)共发生网络分析表明ꎬ艾纳香根和
soils [J]. Microorganismsꎬ 9(8): 1689.
叶中 Cd 积累使内生菌共发生网络变得更加复杂ꎮ HE YMꎬ YANG ZXꎬ LI MRꎬ et al.ꎬ 2017. Effects of a dark
器官 Cd 积累增强了根、茎物种间的竞争关系和叶 septate endophyte (DSE) on growthꎬ cadmium contentꎬ and
物种间的共生关系ꎮ physiology in maize under cadmium stress [J]. Environ Sci
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KURAMSHINA ZMꎬ SMIRNOVA YVꎬ KHAIRULLIN RMꎬ
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inoculated with endophytic strains of Bacillus subtilis
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interactions and detecting keystone species with co ̄ LATIF KAꎬ IHSAN Uꎬ JAVID Hꎬ et al.ꎬ 2016. Regulations of
occurrence networks [J]. Front Microbiolꎬ 5: 219. essential amino acids and proteomics of bacterial endophytes
CHEN Bꎬ LUO Sꎬ WU YJꎬ et al.ꎬ 2017. The effects of the Sphingomonas sp. Lk11 during cadmium uptake [J]. Environ
endophytic bacterium pseudomonas fluorescens sasm05 and Toxicolꎬ 31(7): 887-896.
IAA on the plant growth and cadmium uptake of Sedum LI Hꎬ LOU LYꎬ TANG Bꎬ et al.ꎬ 2022. Dynamic changes of
alfredii Hance [J]. Front Microbiolꎬ 8: 2538. rhizosphere soil bacterial community and nutrients in
CHEN ZHꎬ REN JGꎬ PANG YXꎬ et al.ꎬ 2022. Effects of cadmium polluted soils with soybean ̄corn intercropping
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