Page 178 - 《广西植物》2025年第8期
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ZHANG Xiaohua ꎬ FAN Zhiwei ꎬ ZHU Jiafu ꎬ LIN Li ꎬ ZHANG Rui ꎬ
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XU Shengguang ꎬ MA Huanjin ꎬ ZHANG Jinwen
( 1. College of Agronomy and Life Sciencesꎬ Kunming Universityꎬ Kunming 650214ꎬ Chinaꎻ 2. Guandu District Science and Technology
Achievement Transformation Promotion Centerꎬ Kunming 650214ꎬ Chinaꎻ 3. Institute of Food Cropsꎬ Yunnan
Academy of Agricultural Sciencesꎬ Kunming 650205ꎬ China )
Abstract: In order to understand the mechanism of exogenous methyl jasmonate ( MeJA) and chemical fertilizer
reduction in reducing continuous cropping strawberry obstacles from the perspective of antioxidant systems such as
ascorbic acid ̄glutathione (AsA ̄GSH) cycleꎬ this study planted ‘Hongyan’ strawberry in soil that had been used for
continuous cropping of strawberry for four yearsꎬ and measured the nitrate reductase (NR) activityꎬ nitric oxide (NO)
contentꎬ as well as the non ̄enzymatic antioxidant content and related enzyme activity of the AsA ̄GSH cycle. The results
were as follows: (1) Under the condition of conventional chemical fertilizer applicationꎬ exogenous MeJA significantly
increased the NO content in continuous cropping strawberry by 35.31%ꎬ significantly decreased the AsA content by
19.70%ꎬ significantly decreased the ascorbic acid peroxidase (APX) activity by 6.60%ꎬ significantly increased the
economic yield by 6.41%ꎬ and significantly increased biomass by 7.71%. Howeverꎬ it did not significantly affect NR
activityꎬ GSH contentꎬ and glutathione reductase (GR) activity. (2) Under the condition of halving the amount of
chemical fertilizerꎬ exogenous MeJA significantly increased the NO content of continuous cropping strawberry by 53.
31%ꎬ significantly reduced the AsA content by 45.45%ꎬ significantly reduced APX activity by 9.23%ꎬ significantly
increased economic yield by 6.66%ꎬ and significantly increased biomass by 8.17%. Howeverꎬ it did not significantly
affect NR activityꎬ GSH contentꎬ or GR activity. (3) Under clear water sprayꎬ chemical fertilizer reduction significantly
increased the NO contentꎬ AsA contentꎬ and APX activity of continuous cropping strawberry by 27.34%ꎬ 86.11%ꎬ and
6.88%ꎬ respectively. Howeverꎬ it did not significantly affect NR activityꎬ GSH contentꎬ or GR activityꎬ as well as
economic yield and biomass. (4) Under the condition of spraying MeJAꎬ chemical fertilizer reduction significantly
increased the NO content and AsA content in continuous cropping strawberry by 44.27% and 26.42%ꎬ respectively.
Howeverꎬ it did not significantly affect NR activityꎬ GSH contentꎬ APX activityꎬ or GR activityꎬ as well as economic
yield and biomass. In summaryꎬ both exogenous MeJA and chemical fertilizer reduction have no significant effect on the
GSH content and GR activity of continuous cropping strawberry. Howeverꎬ exogenous MeJA can alleviate the stress
response of strawberry plants to continuous cropping and high salt stressꎬ thus reducing the AsA content and APX
activity. Chemical fertilizer reduction significantly increases the AsA content and APX activityꎬ indicating that
continuous cropping strawberry may mainly rely on the AsA pathway to cope with continuous cropping obstacles and
excessive fertilizer stress.
Key words: continuous cropping obstaclesꎬ ascorbic acid ̄glutathione cycleꎬ ascorbic acid peroxidaseꎬ glutathione
reductaseꎬ nitrate reductase
草 莓 ( Fragaria × ananassa ) 是 蔷 薇 科 植物自毒物质分泌积累和土传病害增加等问题突
(Rosaceae)草莓属(Fragaria) 多年生草本植物( 张 出ꎬ致使草莓连作障碍日益严重ꎬ出现严重减产甚
镇武等ꎬ2024)ꎮ 随着设施草莓栽培技术逐渐成 至绝收(杨馥霞等ꎬ2023ꎻ李荣飞等ꎬ2023)ꎮ
熟ꎬ我国 草 莓 产 业 发 展 迅 猛 ( 田 春 丽 等ꎬ2024)ꎬ 抗坏血酸-谷胱甘肽(ascorbic acid ̄glutathioneꎬ
2021 年草莓栽培总面积达 1.29× 10 hm ꎬ总产量 AsA ̄GSH)循环在植物应对连作胁迫引发的氧化
2
5
达 3.39×10 tꎬ产值超过 2.50×10 元ꎬ是世界最大 损伤中发挥着关键作用ꎮ 在连作胁迫条件下ꎬ土
10
6
的草莓生产和消费国(吴娥娇等ꎬ2023)ꎮ 但是ꎬ近 壤中因持续种植相同作物而积累的有害物质ꎬ如
年来草莓规模化设施生产导致倒茬轮作困难( 樊 病原微生物及根系分泌的酚酸类自毒物质( 赵振
娅萍等ꎬ2024)ꎬ常年连作造成的土壤盐渍化严重、 利等ꎬ2023ꎻ赵忆南等ꎬ2024)ꎬ能够引发植物过量

