Page 17 - 《广西植物》2026年第6期
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9 3 2                                  广  西  植  物                                         46 卷
              ( 1. School of Soil and Water Conservationꎬ Jiangxi University of Water Resources and Electric Powerꎬ Nanchang 330099ꎬ Chinaꎻ 2. Jiangxi
                    Provincial Industry Technology Engineering Center for Seed ̄Breeding and Utilization of Camphor Treesꎬ Nanchang 330099ꎬ
                                 Chinaꎻ 3. Ji’an Landscape and Gardening Centerꎬ Ji’an 343000ꎬ Jiangxiꎬ China )


                 Abstract: To investigate the community diversity of endophytic fungi in three citral chemotype tree species in
                 Camphoraꎬ and to explore the diversity and structural differences of endophytic fungi in different tree species with the
                 same chemotype and various tissue partsꎬ this study analyzed 36 samples by high ̄throughput sequencing methodꎬ and a
                 total of 2 207 878 sequences were obtained. The results were as follows: (1) The endophytic fungal communities of
                 different tree species with the citral chemotype in Camphora exhibited significant differences in species compositionꎬ and
                 the endophytic fungal species composition showed organ preference and diversity in rootsꎬ stemsꎬ and leaves. (2) The
                 ranking of the Chao and Ace indices was consistentꎬ both showing Camphora officinarum ( QCA) > C. bodinieri
                 (QBO) > C. parthenoxylon (QPO)ꎬ indicating that C. officinarum had the highest species richness of endophytic fungiꎬ
                 with 690 OTUsꎬ while C. parthenoxylon had the lowest species richnessꎬ with 414 OTUs. In contrastꎬ the Shannon diversity
                 index showed the order QPO > QBO > QCAꎬ and the Simpson diversity index exhibited the opposite trend. Thusꎬ it could
                 be concluded that the endophytic fungal community diversity of C. officinarum was the lowest. (3) Principal coordinate
                 analysis (PCoA) based on Bray ̄Curtis distances revealed that the sample points of C. bodinieri and C. parthenoxylon were
                 closely clusteredꎬ indicating a high similarity in their endophytic fungal community structures. In comparisonꎬ the sample
                 points of these two species were relatively dispersed from those of C. officinarumꎬ suggesting a lower similarity in endophytic
                 fungal community structure between them and C. officinarum. (4) The three citral chemotype tree species of Camphora all
                 exhibited similar community structures of endophytic fungi in leaves and stemsꎬ but showed significant differences in the
                 fungal communities between roots and leaves. The results indicate that the combined influence of host tree species and tissue
                 heterogeneity shapes the endophytic fungal communityꎬ laying a foundation for exploring potential functional endophytic
                 fungi in citral chemotype tree species of Camphora and provide insights into the co ̄evolutionary relationship between
                 endophytic fungal communities and host tree species and chemotypes.
                 Key words: chemotype of host speciesꎬ endophytic fungiꎬ high ̄throughput sequencingꎬ species diversityꎬ
                 community composition




                樟属( Camphora) 植物是重要的经济林木ꎬ其                     内生真菌群落结构及其与宿主关系的差异性研究
            中部分树种因富含特定萜类化合物而被划分为不                              已较多ꎬ但目前主要聚焦于木本植物的不同树种
            同的化学型ꎬ根据其所含特征性萜类化合物的差                              (Sun et al.ꎬ 2012ꎻLi et al.ꎬ 2016ꎻ钱海源等ꎬ2018)
            异ꎬ可划分为多种化学型ꎬ如樟脑型、芳樟醇型和                             及相同树种不同季节( Elmagzob et al.ꎬ 2019)、不
            柠檬醛型等( 郑永杰ꎬ2022)ꎮ 柠檬醛是一种含香                         同地区( Fang et al.ꎬ 2019ꎻ杨娟等ꎬ2019ꎻ周思旋
            叶醛和橙花醛两种同分异构体的单萜醛类化合物                              等ꎬ2023)或不同组织部位( 顾美英等ꎬ2022ꎻLi et
            (Ling et al.ꎬ 2022)ꎬ是樟属的一种化学型ꎬ也是合                  al.ꎬ 2023)ꎮ 近年来的研究表明ꎬ内生真菌能够参
            成维生素 A、维生素 E 和紫罗兰酮的关键原料ꎬ广                          与或 调 控 植 物 萜 类 等 次 生 代 谢 产 物 的 合 成

            泛应用于医药、食品、香精香料等领域(Thielmann &                      (Kellerꎬ 2019ꎻ毛欣颖等ꎬ2025)ꎻ同一树种不同化
            Muranyiꎬ 2019ꎻ向育君等ꎬ2020)ꎮ                          学型的内生真菌群落结构也具有明显的差异性
                 内生真菌( endophytic fungi) 是指在其生活史               (吕立新等ꎬ2014)ꎻ植物内生真菌群落结构的差异
            的一定或全部阶段生活于健康植物各种组织或细                              既存在于不同宿主间ꎬ也存在于同一宿主的不同
            胞间隙且不引起明显疾病症状的真菌ꎬ其与宿主                              组织间(王涛等ꎬ2009)ꎻ即使宿主植物相同ꎬ也会
            植物通过长期的协同进化形成了独特的互惠共生                              因不同组织中活性物质的组成和生态位的差异而
            关系(Gakuubi et al.ꎬ 2021)ꎮ 它们经长期协同进                 显示出组织特异性(王营等ꎬ2017)ꎮ 因此ꎬ揭示相
            化广泛分布于植物各器官ꎬ其种类、数量和分布特                             同化学型树种的内生真菌群落的组成与多样性ꎬ
            征均因植物种类而异( 郭顺星ꎬ2018)ꎮ 尽管植物                         对于理解相同化学型下不同树种间菌群的共性与
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