荞麦属种质资源发芽种子酯酶同工酶研究

Esterase isozyme on sprouting seeds of genus Fagopyrum

关键词:  甜荞  苦荞  野荞  酯酶同工酶  系统关系

Key words:  common buckwheat  tartary buckwheat  wild buckwheat  esterase isozyme  phylogeny

摘要:

用聚丙烯酰胺凝胶电泳对荞麦属8个种(含大粒组7个和小粒组1个)33份材料发芽种子的酯酶同工酶进行了研究。结果表明:酯酶同工酶不同酶带合计22条,不同物种的酶带数4到8条。其中,甜荞有8条带,苦荞为7条。酶带及聚类分析表明,大粒组荞麦种的谱带与细野荞等小粒组荞麦种间差异极大,甜荞和苦荞酶带分别与大野荞和毛野荞相似,并分别与F.megaspartanium和F.pilus聚类最近,支持F.megaspartanium和F.pilus可能分别是甜荞和苦荞祖先种的假说。

Abstract:

The esterase isozyme of thirty-three collections of cultivated and wild buckwheat belonging to eight species of genus Fagopyrum including seven species in the big-achene group and one species in the small-achene group were studied by means of polyacrylamide gel eletrophoresis(PAGE). The results showed that there were twenty-two different bands of esterase isozyme,with a range from four bands to eight bands among different buckwheat species. F.esculentum and F.tataricum had eight bands and seven bands,respectively. The variations of esterase isozyme zymograph were much great among different buckwheat species but less in the same species. The results of the zymograph analysis and the clustering showed that there were great differences of zymographs between the big-achene group and the small-achene group and that F.megaspartanium and F.pilus were close in clustering tree to F.esculentum and F.tataricum,respectively,supporting Chen's hypothesis that F.megaspartanium and F.pilus might be the ancestor species of F.esculentum and F.tataricum,respectively.