红麻UG93细胞质雄性不育系、保持系及恢复系间的遗传多样性分析

Genetic diversity in kenaf(Hibiscus cannabinus)UG93 cytoplasmic male sterile lines,maintainer lines and restorer lines revealed by ISSR and cpSSR markers

关键词:  ISSR  cpSSR  细胞质雄性不育(CMS)  遗传多样性

Key words:  ISSR  cpSSR  Cytoplasmic male sterility(CMS)  genetic diversity

摘要:

利用8条核基因组ISSR引物和7对叶绿体基因组SSR引物(cpSSR),对9对红麻UG93细胞质雄性不育系/保持系及5个恢复系的细胞核、细胞质遗传多样性进行分析。结果表明:各材料的核基因组遗传相似系数在0.333～1.000之间,其中保持系间、保持系和恢复系间、恢复系间的平均相似系数分别为0.583、0.689和0.812。质基因组的遗传相似系数在0～0.286之间,23个材料被聚类为4种单倍型,其中9个不育系单独聚为一类。9对不育系/保持系中有5对在ISSR分析中出现差异带,表明这5对不育系/保持系间未发生完全的核置换。本研究结果对创造新的不育资源、组配优良杂交组合具有重要意义。

Abstract:

8 ISSR primers and 7 chloroplast SSR(cpSSR)primer pairs were employed to study genetic diversity among 9 pairs of kenaf UG93 cytoplasmic male sterile/maintainer lines and 5 restorer lines. Nucleus genome genetic similarity coefficients(SC)based on ISSR ranged from 0.333 to 1.000,with the average of 0.583,0.689 and 0.812 among maintainer lines,maintainer and restorer lines,and restorer lines respectively. Chloroplast genome SC based on cpSSR ranged from 0 to 0.286. The 23 tested lines were clustered into 4 haplotypes,with all 9 sterile lines were classified into a single type. 5 of 9 couple of sterile/maintainer lines showed some degree diversity which indicated that replacement of nucleus genome between sterile line and maintainer line was not complete. The results will help generating new kenaf sterile resource and excellent cross combinations.