蓖麻根提取物对HepG2、NCI H460和SGC 7901细胞增殖及凋亡作用的影响

Effects of Ricinus communis Root Extract on proliferation and Apoptosis of HepG2， NCI H460 and SGC 7901 cell

关键词:  蓖麻根提取物  MTT  肿瘤细胞株  细胞凋亡

Key words:  Ricinus communis  MTT  tumor cell  apoptosis

摘要:

探讨蓖麻根不同提取物对肝癌HepG2细胞株、肺癌NCI H460细胞株和胃癌SGC 7901细胞株增殖及其凋亡的影响。采用MTT法检测蓖麻根不同提取物处理48h、72h对HepG2细胞、NCI H460细胞和SGC 7901细胞增殖的抑制率；Hoechst 33258荧光染料染色法观察HepG2细胞凋亡，流式细胞术检测HepG2细胞周期。结果表明：蓖麻根石油醚提取物对HepG2细胞、NCI H460细胞和SGC 7901细胞增殖有较强抑制作用，48 h的IC50分别为88.6、134.3 、138.1 μg/mL，72 h的IC50分别为65.6、133.3、136.6 μg/mL；乙酸乙酯提取物对HepG2细胞、NCI H460细胞和SGC 7901细胞增殖在72h有中等强度抑制作用，IC50分别为90.2、138.5、188.2 μg/mL；氯仿提取物对NCI H460细胞和SGC 7901细胞增殖抑制作用弱，对HepG2细胞增殖基本无抑制作用；Hoechst 33258荧光染色显示石油醚提取物60 μg/mL可使HepG2细胞出现凋亡细胞，流式细胞术检测显示石油醚提取物60 μg/mL可将HepG2细胞阻滞于S期（与对照组比较P＜0.05）。

Abstract:

Effects of Ricinus communis root extracts on proliferation and apoptosis in human hepatoma cell lines HepG2，lung cancer cell lines NCI H460 and gastric cancer cell lines SGC 7901 were investigated. Cell proliferation rate of different root extracts on HepG2 cells，NCI H460 cells and SGC 7901 cells was determined by MTT assay. The apoptosis of HepG2 cells was observed by fluorescent dye staining with Hoechst 33258. HepG2 cell cycle was measured by flow cytometry. The results showed that petroleum ether extract of R.communis root had a strong inhibitory effect on proliferation of HepG2 cells，NCI H460 cells and SGC 7901 cells，the IC50 respectively were 88.6，134.3 and 138.1 g/mL in 48h，the IC50 were respectively 65.6，133.3 and 136.6 μg/mL in 72h. Ethyl acetate extract also had a moderate inhibitory effect on proliferation of HepG2 cells，NCI H460 cells and SGC 7901 cells in 72 h，the IC50 respectively were 90.2，138.5 and 188.2 μg/mL. Chloroform extract had mild intensity to the proliferation of NCI H460 cells and SGC 7901 cells in the 72 h，but chloroform extract had no inhibition to HepG2 cell proliferation. The Hoechst 33258 fluorescence dyeing demonstrated that petroleum ether extract(60 μg/mL) could make the HepG2 cell appears apoptosis. Flow cytometry analysis showed that petroleum ether extract (60 μg/mL) could arrest HepG2 cells in S phase(compared with control group，P＜0.05).