| 引用本文: | 马湘玮, 朱鹏锦, 杜英俊, 宋奇琦, 叶维雁,
唐秀观, 何 江, 钟云婕, 欧景莉, 庞新华.菠萝蜜WRKY基因家族注释及在低温胁迫下的表达模式分析[J].广西植物,2025,45(5):903-915.[点击复制] |
| MA Xiangwei, ZHU Pengjin, DU Yingjun, SONG Qiqi, YE Weiyan,
TANG Xiuguan, HE Jiang, ZHONG Yunjie, OU Jingli, PANG Xinhua.Identification and expression pattern analysis of WRKY gene family in jackfruit under low temperature stress[J].Guihaia,2025,45(5):903-915.[点击复制] |
|
| |
|
|
| 本文已被：浏览 338次 下载 129次 |
码上扫一扫！ |
|
|
| 菠萝蜜WRKY基因家族注释及在低温胁迫下的表达模式分析 |
|
马湘玮1,2, 朱鹏锦1,2*, 杜英俊1,2, 宋奇琦1,2, 叶维雁1,2,
唐秀观1,2, 何 江1,2, 钟云婕1,2, 欧景莉1,2, 庞新华1,2
|
|
1. 广西壮族自治区亚热带作物研究所, 南宁 530000;2. 亚热带特色水果质量安全控制重点实验室, 南宁 530000
|
|
| 摘要: |
| WRKY转录因子家族在植物生物和非生物胁迫响应过程中发挥着重要作用,该研究基于菠萝蜜全基因组,共注释出61个菠萝蜜(Artocarpus heterophyllus)WRKY基因家族成员,并利用生物信息学方法和定量实时荧光 PCR(qRT-PCR)技术分析WRKY基因家族成员在不同品种菠萝蜜低温胁迫下的表达特征。结果表明:(1)进化树分析表明,菠萝蜜、拟南芥(Arabidopsis thaliana)和水稻(Oryza sativa)WRKY基因家族成员被分为4个亚族。(2)染色体定位结果显示,61个菠萝蜜WRKY基因家族成员不均匀地分布在23条染色体上。(3)保守基序和基因结构分析表明,位于同一亚族的菠萝蜜WRKY基因家族成员具有相似的保守基序和基因结构。(4)进化分析显示,菠萝蜜WRKY基因家族成员在物种内存在124对片段复制基因对; 物种间分析表明,菠萝蜜与拟南芥之间的同源基因对较多,而与水稻和无花果(Ficus carica)之间的同源基因对较少且有8个基因在3个物种中形成同源基因对。(5)低温胁迫下不同品种菠萝蜜转录组分析结果表明,菠萝蜜WRKY基因家族成员在不同品种中的表达模式存在差异,qRT-PCR分析进一步验证这一结果,说明WRKY基因家族成员在不同品种菠萝蜜响应低温胁迫的过程中发挥重要作用。该研究为了解WRKY基因家族成员的进化和功能提供了新的见解,为菠萝蜜WRKY基因家族成员的功能研究和利用奠定了基础。 |
| 关键词: 菠萝蜜, WRKY基因家族, 低温胁迫, 表达模式, 生物信息学分析 |
| DOI：10.11931/guihaia.gxzw202405046 |
| 分类号:Q943 |
| 文章编号:1000-3142(2025)05-0903-13 |
| 基金项目:广西重点研发计划(桂科AB21220003); 广西农科院基本科研业务专项(桂农科2023YM15); 广西农业科技创新联盟项目(桂农科盟202404)。 |
|
| Identification and expression pattern analysis of WRKY gene family in jackfruit under low temperature stress |
|
MA Xiangwei1,2, ZHU Pengjin1,2*, DU Yingjun1,2, SONG Qiqi1,2, YE Weiyan1,2,
TANG Xiuguan1,2, HE Jiang1,2, ZHONG Yunjie1,2, OU Jingli1,2, PANG Xinhua1,2
|
|
1. Guangxi Subtropical Crops Research Institute, Nanning 530000, China;2. Guangxi Key Laboratory
of Quality and Safety Control for Subtropical Fruits, Nanning 530000, China
1. Guangxi Subtropical Crops Research Institute, Nanning 530000, China; 2. Guangxi Key Laboratory
of Quality and Safety Control for Subtropical Fruits, Nanning 530000, China
|
| Abstract: |
| The WRKY transcription factors family plays an important role in plant biological and abiotic stress responses, a total of 61 WRKY gene family members of that jackfruit(Artocarpus heterophyllus)were identified based on the whole genome of jackfruit and the expression profile of WRKY gene family members in jackfruit under low temperature stress were analyzed by bioinformatics method and quantitative real-time fluorescence PCR(qRT-PCR). The results were as follow:(1)Phylogenetic tree analysis showed that WRKY gene family members of jackfruit, Arabidopsis thaliana and rice(Oryza sativa)were divided into four subfamilies.(2)Chromosome localization showed that 61 AhWRKY gene family members were unevenly distributed on 23 chromosomes.(3)Analyses of conserved motifs and gene structure showed that AhWRKY gene family members located in the same subfamily had similar conserved motifs and gene structure.(4)Phylogenetic analysis revealed 124 pairs of fragment duplicated genes within AhWRKY gene family members; inter-species analysis showed jackfruit exhibits a greater number of homologous gene pairs with Arabidopsis thaliana than rice and fig(Ficus carica), and 8 genes formed homologous gene pairs in Arabidopsis thaliana, rice and fig.(5)Transcriptomic analysis of different varieties of jackfruit under low temperature stress showed that there the expression patterns of WRKY gene family members differed in different varieties. Moreover, qRT-PCR analysis further verified this result, indicating that WRKY gene family members play a role in response to low temperature stress in jackfruit of different varieties. This study provides new insights into the evolution and function of WRKY gene family members, and lays a foundation for functional research and utilization of WRKY gene family members in jackfruit. |
| Key words: jackfruit, WRKY gene family, low temperature stress, expression pattern, bioinformatics analysis |
|
|
|
|
|
