云南三种特色柑橘属果皮精油成分及活性研究

李 渔<sup>2</sup>; 陈 波<sup>3</sup>; 施建莲<sup>4</sup>; 马海玲<sup>1</sup>; 习杨彦彬<sup>5</sup>; 刘 佳<sup>1*</sup>

引用本文:	李渔, 陈波, 施建莲, 马海玲, 习杨彦彬, 刘佳.云南三种特色柑橘属果皮精油成分及活性研究[J].广西植物,2022,42(9):1507-1513.[点击复制]
	LI Yu, CHEN Bo, SHI Jianlian, MA Hailing, XI Yangyanbin, LIU Jia.Components and activities of essential oils from peel of three endemic Citrus L. in Yunnan[J].Guihaia,2022,42(9):1507-1513.[点击复制]

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云南三种特色柑橘属果皮精油成分及活性研究
李渔², 陈波³, 施建莲⁴, 马海玲¹, 习杨彦彬⁵, 刘佳^1*
1. 昆明医科大学海源学院, 昆明 650101;2. 云南省药品评价中心, 昆明 650101;3. 昆明医科大学科技成果孵化中心, 昆明 650500;4. 云南新兴职业学院, 昆明 650501;5. 昆明医科大学神经科学研究所, 昆明 650500

摘要:

为探究云南特色柑橘属西双版纳曼赛龙柚、元江绿皮冰糖橙、德宏青柠的果皮精油成分及抗肿瘤活性。该研究采用水蒸气蒸馏法提取西双版纳曼赛龙柚、元江绿皮冰糖橙、德宏青柠果皮的精油,通过GC-MS开展定性定量成分分析,并通过MTT法开展果皮精油抑制肿瘤增殖研究。结果表明:德宏青柠果皮精油提取率最高、元江绿皮冰糖橙果皮精油次之、西双版纳曼赛龙柚果皮精油最低; 从西双版纳曼赛龙柚、元江绿皮冰糖橙、德宏青柠的果皮精油中分别鉴定出24种、11种、13种成分,月桂烯、萜品烯是三种精油的共有成分。西双版纳曼赛龙柚和元江绿皮冰糖橙的果皮精油中都含有β-蒎烯、3,7-二甲基-1,6辛二烯-3-醇、柠檬烯、α-松油醇,以及重要香料成分乙酸二甲基苄基原酯和二氢茉莉酮酸甲酯,其中西双版纳曼赛龙柚精油中柠檬烯含量高达72.097%,德宏青柠精油中含有活性成分柠檬醛。三种精油对胃癌细胞MKN-45、人乳腺癌细胞MCF-7和结直肠癌细胞HCT-116均有一定的抗肿瘤活性,并呈剂量依赖关系。该研究结果可为云南三种特色柑橘属植物的开发和应用提供科学依据。

关键词: 柑橘属, 果皮, 精油, 气相色谱-质谱, 活性, 噻唑蓝

DOI：10.11931/guihaia.gxzw202011046

分类号:Q946

文章编号:1000-3142(2022)09-1507-07

基金项目:国家自然科学基金(81560238); 云南省科技厅项目(2018FE001-193); 云南省天然药物药理重点实验室开放研究基金(70120030508)[Supported by National Natural Science Foundation of China(81560238); Project of Yunnan Science and Technology Department(2018FE001-193); Open Research Foundation of Yunnan Provincial Key Laboratory of Pharmacology for Natural Products(70120030508)]。

Components and activities of essential oils from peel of three endemic Citrus L. in Yunnan

LI Yu², CHEN Bo³, SHI Jianlian⁴, MA Hailing¹, XI Yangyanbin⁵, LIU Jia^1*

1. Kunming Medical University Haiyuan College, Kunming 650101, China;2. Yunnan Drug Evaluation Center, Kunming 650101, China;3. Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming 650500, China;4. Yunnan Xinxing Occupations College, Kunming 650501, China;5. Insititute of Neuroscience, Kunming Medical University, Kunming 650500, China 1. Kunming Medical University Haiyuan College, Kunming 650101, China; 2. Yunnan Drug Evaluation Center, Kunming 650101, China; 3. Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming 650500, China; 4. Yunnan Xinxing Occupations College, Kunming 650501, China; 5. Insititute of Neuroscience, Kunming Medical University, Kunming 650500, China

Abstract:

In order to investigate and analyze constituents of essential oils from peels and anti-tumor activities of Citrus maxima cv. Mansailong from Xishuangbanna,C. sinensis Osbeck from Yuanjiang, and C. aurantiifolia from Dehong in Yunnan. Essential oils of peels of C. maxima cv. Mansailong from Xishuangbanna,C. sinensis Osbeck from Yuanjiang, and C. aurantiifolia from Dehong were extracted by steam distillation, qualitative and quantitative component analysis were carried out by GC-MS, and the inhibition of tumor proliferation by pericarp essential oil was studied by MTT method. The results were as follows: the extraction rate of essential oil from C. aurantiifolia was the highest, followed by that from C. sinensis Osbeck, and the lowest from C. maxima cv. Mansailong. And 24, 11 and 13 components were identified from essential oils of peels of C. maxima cv. Mansailong from Xishuangbanna, C. sinensis Osbeck from Yuanjiang, and C. aurantiifolia from Dehong, respectively. Myrcene and terpinene were the common components of the three essential oils. Both C. maxima cv. Mansailong and C. sinensis Osbeck essential oil contained active monomers compounds in β-pinene and 3, 7-dimethyl-1, 6-octadiene-3-alcohol, limonene, α-terpineol, and important spice ingredients, dimethyl benzyl acetate and methyl dihydrojasmonate, C. maxima cv. Mansailong essential oil contained limonene up to 72.097%, citral was the active ingredient in C. aurantiifolia from Dehong essential oil. Three essential oils showed certain anti-tumor activities against MKN-45 cell line of gastric cancer, MCF-7 cell line of human breast cancer and HCT-116 cell line of colorectal cancer in a dose-dependent manner. The results provide scientific reference for the development and utilization of the three endemic Cirtus L. in Yunnnan.

Key words: Citrus L., peel, essential oil, GC-MS, activities, MTT