广西地不容非药用部位化学成分研究

Chemical constituents from non-medicinal parts of Stephania kwangsiensis

关键词:  广西地不容, 化学成分, 结构鉴定, 生物碱, 抑菌活性

Key words:   Stephania kwangsiensis, chemical constituentes, structural identification, alkaloid, antibacterial activity

摘要:

广西地不容(Stephania kwangsiensis)为防己科藤本植物,主要分布于广西,其药用部位(块根)中药名为“金不换”,有镇静、杀虫、抗炎、抗肿瘤、抗病毒、抑菌的作用,用于治疗阿尔茨海默病。为了充分利用该植物资源,明确广西地不容非药用部位中的化学成分,并发现其相关的活性成分。该研究综合运用正相Sephadex LH-20、正相硅胶、正相聚酰胺、MCI gel CHP-20P等多种柱色谱法对广西地不容非药用部位甲醇提取物进行分离纯化,所得单体化合物的结构经MS、¹H-NMR、¹³C-NMR等波谱学数据分析及相关文献对照进行鉴定,同时用滤纸法对分离得到的化合物开展抗细菌(大肠杆菌、金色葡萄球菌、枯草芽孢杆菌)和抗真菌(尖孢镰刀菌)的活性评价。结果表明:(1)从该植物中分离得到的10个化合物分别鉴定为吐叶醇(1)、蚱蜢酮(2)、异紫堇定碱(3)、2-O-β-D-glucopyranosyl-D-arabinitol(4)、(9S,12S,13S)-trihydroxyoctadeca-(10E,15Z)-dienoic acid(5)、magnoflorine(6)、corchoionoside C(7)、委陵菜酸(8)、紫堇定碱(9)、克班宁(10)。其中,化合物1、2、4、5、6、7、8为首次从该植物中分离得到,化合物1、2、4、5、7、8为首次从该属植物中分离得到,化合物4为首次从天然产物中分离得到。(2)在化合物浓度为6 mg·mL^-1时,化合物1、2、5对大肠杆菌具有弱抑菌作用,化合物2对金色葡萄球菌有弱的抑菌活性,化合物3和化合物8对枯草芽孢杆菌有弱的抑制作用。综上表明,广西地不容非药用部位含有丰富的活性成分,具有开发利用的潜力。

Abstract:

Stephania kwangsiensis, a vine plant of the Stephaniaceae family, is mainly spread in Guangxi, China. The medicinal part(root tuber)of S. kwangsiensis, “Jin Bu Huan” in Chinese, is often used for sedative, insecticidal, anti inflammatory, anti-tumor, antiviral and antibacterial, as well as for the treatment of Alzheimer's disease. In order to make full use of the plant resources, clarify its chemical constituents, and find the relevant active compounds, the methanol extracts of non-medicinal parts of this plant were isolated using various chromatographic methods such as positive phase Sephadex LH-20, positive phase silica gel, positive phase polyamide, and MCI gel CHP-20P. The structures of the isolated monomer compounds were determined by MS, ¹H-NMR, ¹³C-NMR, and other spectral data analysis, as well as comparison with relevant literatures. The activities of the isolated compounds from S. kwangsiensis against bacteria(Escherichia coli, Staphylococcus aureus, Bacillus subtilis)and fungi(Fusarium oxysporum)were evaluated using filter paper method. The results were as follows:(1)A total of 10 compounds obtained from the non-medicinal parts of this plant were identified as vomifoliol(1), grasshopper ketone(2), isocorydine(3),2-O-β-D-glucopyranosyl-D-arabinitol(4),(9S,12S,13S)-trihydroxyoctadeca-(10E,15Z)-dienoic acid(5), magnoflorine(6), corchoionoside C(7), tormentic acid(8), corydine(9)and crebanine(10). Among them, compounds 1, 2, 4, 5, 6, 7 and 8 were isolated from this plant for the first time, compounds 1, 2, 4, 5, 7 and 8 were firstly obtained from this genus plants, and Compound 4 was found from natural products for the first time.(2)At concentration of 6 mg·mL^-1, compounds 1, 2 and 5 showed weak antibacterial activities against Escherichia coli; Compound 2 exhibited weak antibacterial activities against Staphylococcus aureus; compounds 3 and 8 displayed weak antibacterial activities against Bacillus subtilis. In conclusion, the non-medicinal parts of Stephania kwangsiensis contain large numbers of active components and have the potential of development and utilization.