| 摘要: |
| 该研究采用浸提法和GC-MS检测了贵州石笔木种子内源有机化合物种类、相对含量,以及种皮和胚乳在不同溶剂、温度和浓度条件下的浸提物活性。结果表明:(1)种皮和胚乳中皆含有有机酸、烯、酯、醇、醛、酚6类相对含量较高的有机化合物,其中种皮含有机酸7种、烯类1种、酯类5种、醇类3种、醛类3种、酚类1种,胚乳含有机酸6种、烯类1种、酯类1种、醇类1种、醛类1种、酚类1种。(2)种皮浸提物活性显著高于胚乳浸提物活性(P<0.05),且其烯、醛、醇和酯类含量分别高出胚乳含量的8.78%、2.66%、2.15%和1.70%,可能是对种子萌发起主要作用的内源抑制物质。(3)不同条件下处理的浸提液均能显著抑制白菜种子发芽及幼苗生长,浸提液抑制物活性表现为醇溶剂大于水溶剂,并随着浸提液浓度的升高而增大、随着浸提温度的升高而增强,在初始温度为100 ℃时,浸提液抑制活性达到最大值。贵州石笔木种子的内源有机化合物在种子萌发过程中发挥着不同程度的抑制作用,探索其与种子萌发的作用机制,解决种子萌发育苗的关键技术及在农林业生产中应用,这在植物种子的生物学特性及萌发生理研究方面具有重要意义。 |
| 关键词: 种子活力, 种子内源抑制物, GC-MS鉴定, 萌发生理, 贵州石笔木 |
| DOI：10.11931/guihaia.gxzw201811008 |
| 分类号: |
| 文章编号:1000-3142(2019)11-1558-10 |
| Fund project:国家自然科学基金(31560097); 贵州省科技计划项目(黔科合 [2016]支撑2836号,黔科合成果 [2019]4237号)[Supported by the National Natural Science Foundation of China(31560097); the Science and Technology Support Program of Guizhou Science and Technology Department(2016-2836)& Achievement Transformation(2019-4237)]。 |
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| Effects of endogenous inhibitors and their activities onseed germination of Tutcheria kweichowensis |
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LV Xiaomei 1, ZOU Tiancai 2 , WANG Jianwen 2, HONG Jiang2, LIU Haiyan 3 *, GUO Mixiang 4
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1.Nanpanjiang State Forestry Farm, Buyi and Miao Autonomous Prefecture, Southwest Guizhou, Xingyi 562400, Guizhou, China;2. Guizhou Academy of Sciences, Guiyang 550001, China;3. Guizhou Botanical Garden, Guiyang 550004, China;4. College of Forestry, Guizhou University, Guiyang 550025, China
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| Abstract: |
| The components and contents of endogenous inhibitors in seed germination of Tutcheria kweichowensis were identified by extraction method and GC-MS, as well as the activity of extracts from seed coat and endosperm in different solvents, temperatures and concentrations. The results were as follows:(1)The seed coat and endosperm contained six kinds of organic compounds, such as organic acids, olefins, esters, alcohols, aldehydes, phenols and so on. The seed coat contained 7 organic acids, 1 alkene, 5 esters, 3 alcohols, 3 aldehydes and 1 phenol. The endosperm contained 6 organic acids, 1 alkene, 1 ester, 1 alcohol, 1 aldehyde and 1 phenol.(2)The activity of extracts from seed coat was significantly higher than that of endosperm extract(P<0.05), and the contents of olefins, aldehydes, alcohols and esters were 8.78%, 2.66%, 2.15% and 1.70% higher than that of endosperm.(3)The extracts from different conditions could significantly inhibit the germination and seedling growth of Brassica pekinensis. The activity of extract inhibitors was higher in alcohol than in water, and increased with the increase of extract concentration and temperature. When the initial temperature was 100 ℃, the inhibitory activity of the extract reached the maximum. The endogenous inhibitors of Tutcheria kweichowensis have different inhibitory effects on seed germination, exploring its mechanism of seed germination, solving the key technology of seed germination and seedling and application in agricultural and forestry production, which is of great significance in the research of biological characteristics and germination physiology of plant seeds. |
| Key words: seed vitality, endogenous inhibitors of seeds, GC-MS identification, germination physiology, Tutcheria kweichowensis |
