植物激素和接种方式对广西金线莲壮苗生根的影响

Effects of phytohormone and inoculation method on cultivating strong seedling and inducing root of Guangxi Anoectochilus roxburghii

关键词:  植物激素, 接种方式, 广西金线莲, 组培苗, 壮苗生根

Key words:  phytohormone, inoculation method, Guangxi Anoectochilus roxburghii, in vitro shoots, cultivating strong seedling and inducing root

摘要:

该文以广西野生金线莲无菌播种离体茎段为材料,采用单因素对比试验,研究了植物激素(NAA、IBA、6-BA、GA₃、KT、ZT、TDZ、2-IP)以及接种方式(竖直接种和水平接种)对壮苗生根培养的影响。结果表明:与对照相比,生长素有利于壮苗生根,NAA的效果优于IBA; 细胞分裂素对壮苗生根的效果依次为6-BA>TDZ>KT=ZT>2-IP>CK,其中6-BA诱导平均株高8.4 cm,3.6条根,茎粗为2.84 mm,植株生长健壮,诱导效果最好; 赤霉素GA₃诱导出的植株高且直,但植株细弱,且抑制根系生长,不利于壮苗生根培养; 在激素组合6-BA 0.5 mg·L^-1、NAA1.0 mg·L^-1处理中,组培苗生长健壮且根数量较多,效果最佳; 水平接种能诱导出更多的根系,且便于接种操作,可以节省接种时间。因此,确定广西金线莲最适宜的壮苗生根培养基配方为1/2MS+6-BA 0.5 mg·L^-1+NAA 1.0 mg·L^-1+香蕉汁100 g·L^-1+AC(活性炭)1.0 g·L^-1+蔗糖20 g·L^-1,最佳接种方式为水平接种。

Abstract:

Selecting stem segments of wild Guangxi Anoectochilus roxburghii as the study object, single factor comparison was carried out to determine the effects of phytohormone(NAA, IBA, 6-BA, GA₃, KT, ZT, TDZ, 2-IP)and inoculation method(vertical inoculation and horizontal inoculation)on cultivating strong seedling and inducing root. The results showed that compared to control treatment, NAA was better than IBA in cultivating strong seedling and inducing root. And the effects of cytokinins were as 6-BA >TDZ>KT=ZT> 2-IP >CK. The average height of seedlings in 6-BA treatment was 8.4 cm, the average root number was 3.6, the stem diameter was 2.84 mm, all seedlings grown vigorously with the best inducing effect. Gibberellin GA₃ could induced seedlings tall and straight, but it could not make seedling strong and it inhibited the growth of roots. The combination of 6-BA 0.5 mg·L^-1 and NAA 1.0 mg·L^-1 generated a good effect, which could induce strong seedlings and numerous roots. Horizontal inoculation could induce numerous roots, and it was convenient for operation and saved time of inoculation. Therefore, it can be concluded that during the strong plant culture and root inducing of Guangxi Anoectochilus roxburghii, the optimum medium is 1/2MS+6-BA 0.5 mg·L^-1 +NAA 1.0 mg·L^-1+banana mud 100 g·L^-1+ AC 1.0 g·L^-1+ sugar 20 g·L^-1, and the best inoculating method is to inoculate horizontally.