基于ITS序列探讨珍珠菜属过路黄组的系统关系

Phylogenetic relationships of sect.Nummularia (Lysimachia):evidence from ITS sequences

关键词:  珍珠菜属  过路黄组  ITS  系统关系

Key words:  Lysimachia  Nummularia  nrDNA ITS  molecular phylogeny

摘要:

测定了紫脉过路黄、临时救和过路黄的nrDNA ITS序列，并分析了珍珠菜属过路黄组17个物种的遗传距离及亲缘关系。结果表明，过路黄组植物的ITS序列长度在620～628间，一致性高达90.59%，种间遗传距离为0.002～0.199。系统发育树表明：(1)紫脉过路黄、临时救和小茄亲缘关系较近；(2)大叶过路黄、落地梅和过路黄亲缘关系较近；(3)山萝过路黄、贯叶过路黄、管茎过路黄、叶头过路黄、峨眉过路黄及三角叶过路黄亲缘关系较近。ITS序列分析结果为组内植物的鉴定、分类及系统进化提供了新的参考。

Abstract:

The rDNA ITS sequences of L.rubinervis，L.congestiflora and L.christinae were sequenced. The genetic distance and phylogenetic relationship of 17 species from sect.Nummularia(Lysimachia)were analyzed. The results showed that the length of ITS sequence of sect.Nummularia was 620-628 base pairs，the homology was 9059%，and the genetic distance was 0.002-0.199. The ITS data suggested that:(1)L.japonica，L.rubinervis and L.congestiflora were closely related；(2)L.fordiana，L.paridiformis and L.christinae were closely related；(3)L.melampyroides，L.perfoliata，L.fistulosa，L.phyllocephala，L.omeiensis and L.deltoidea had close relationship. The ITS analysis provided new reference for the relationship，identification and classification of the species in sect.Nummularia.