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表 2 花魔芋软腐病块组织分离真菌 ITS 和 LSU 序列 NCBI 数据库比对结果
Table 2 Comparison results of isolation fungal ITS and LSU sequences soft
rot tissue of Amorphophallus konjac in from NCBI database
相似菌株 相似度
菌株编号 序列名称 GenBank 登录号 相似真菌
Identity
Strain No. Sequence name GenBank accession Similar strain
(%)
No. of similar strain
M1 ITS NR111886.1 轮纹镰刀菌 98.27
Fusarium concentricum
LSU NG069847.1 98.85
M3 M4 M6 M9 M11 M14 ITS NR111886.1 98.24
LSU NG069847.1 98.85
M5 ITS NR111886.1 96.89
LSU NG069847.1 98.62
M8 ITS NR111886.1 97.88
LSU NG069847.1 98.77
M25 ITS NR111886.1 98.24
LSU NG069847.1 98.77
M7 ITS LT841236.1 尖孢镰刀菌 98.95
F. oxysporum
LSU LT841236.1 98.95
M10 ITS NR173405.1 F. ambrosium 98.01
LSU NG076685.1 98.92
M15 ITS NR173405.1 97.82
LSU NG076685.1 98.92
M12 ITS NR165993.1 粉红螺旋聚孢霉 99.25
Clonostachys rosea f. catenulata
LSU NG063969.1 99.65
M19 M22 ITS NR165993.1 99.25
LSU NG063969.1 99.77
M17 ITS NR103628.1 拜尼尔毛霉 97.22
Mucor bainieri
LSU NG067371.1 99.23
M21 ITS NR103628.1 91.39
LSU NG067371.1 99.20
M18 ITS NR103653.1 单孢根霉 98.89
Rhizopus azygosporus
LSU NG066155.1 99.00
M20 ITS NR103653.1 98.89
LSU NG066155.1 85.85
M23 ITS NR103653.1 98.73
LSU NG066155.1 98.22
M24 ITS & LSU JX290030.1 氯氮卓青霉 91.80
Penicillium solitum
注: M1、M3~ M12、M14、M15、M17~ M25 为分离菌株编号ꎮ 下同ꎮ
Note: M1ꎬ M3-M12ꎬ M14ꎬ M15ꎬ M17-M25 are the numbers of isolated strains. The same below.
分离出了毛霉、根霉和青霉等腐生真菌ꎬ这些真菌 种多糖降解酶类物质( Lange et al.ꎬ2019)ꎬ从而影
不能引起健康魔芋球茎发病ꎬ它们在软腐病发病 响魔芋球茎健康部位ꎬ加快软腐病的发病速度ꎮ
过程中具体有何作用ꎬ还有待进一步研究ꎮ 但是ꎬ 此外ꎬ我们还从魔芋软腐病状组织病健交界处分
这些腐生真菌可以加速软腐组织的降解(Kavkler & 离出了粉红螺旋聚孢霉属真菌ꎬ它是一种生防菌ꎬ
Demšarꎬ2019)ꎬ在其快速繁殖的过程也会分泌各 能够 捕 食 真 菌 和 线 虫 ( Seenivasagan & Babalolaꎬ