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3 4 0                                  广  西  植  物                                         44 卷
                          表 2  花魔芋软腐病块组织分离真菌 ITS 和 LSU 序列 NCBI 数据库比对结果
                              Table 2  Comparison results of isolation fungal ITS and LSU sequences soft
                                     rot tissue of Amorphophallus konjac in from NCBI database

                                                           相似菌株                                      相似度
                    菌株编号                序列名称            GenBank 登录号              相似真菌
                                                                                                     Identity
                    Strain No.         Sequence name    GenBank accession       Similar strain
                                                                                                     (%)
                                                        No. of similar strain
                       M1                 ITS             NR111886.1             轮纹镰刀菌               98.27
                                                                             Fusarium concentricum
                                          LSU             NG069847.1                                 98.85
               M3 M4 M6 M9 M11 M14        ITS             NR111886.1                                 98.24
                                          LSU             NG069847.1                                 98.85
                       M5                 ITS             NR111886.1                                 96.89
                                          LSU             NG069847.1                                 98.62
                       M8                 ITS             NR111886.1                                 97.88
                                          LSU             NG069847.1                                 98.77
                      M25                 ITS             NR111886.1                                 98.24
                                          LSU             NG069847.1                                 98.77
                       M7                 ITS             LT841236.1             尖孢镰刀菌               98.95
                                                                                F. oxysporum
                                          LSU             LT841236.1                                 98.95
                      M10                 ITS             NR173405.1            F. ambrosium         98.01
                                          LSU             NG076685.1                                 98.92
                      M15                 ITS             NR173405.1                                 97.82
                                          LSU             NG076685.1                                 98.92
                      M12                 ITS             NR165993.1           粉红螺旋聚孢霉               99.25
                                                                           Clonostachys rosea f. catenulata
                                          LSU             NG063969.1                                 99.65
                     M19 M22              ITS             NR165993.1                                 99.25
                                          LSU             NG063969.1                                 99.77
                      M17                 ITS             NR103628.1             拜尼尔毛霉               97.22
                                                                                Mucor bainieri
                                          LSU             NG067371.1                                 99.23
                      M21                 ITS             NR103628.1                                 91.39
                                          LSU             NG067371.1                                 99.20
                      M18                 ITS             NR103653.1             单孢根霉                98.89
                                                                              Rhizopus azygosporus
                                          LSU             NG066155.1                                 99.00
                      M20                 ITS             NR103653.1                                 98.89
                                          LSU             NG066155.1                                 85.85
                      M23                 ITS             NR103653.1                                 98.73
                                          LSU             NG066155.1                                 98.22
                      M24               ITS & LSU         JX290030.1             氯氮卓青霉               91.80
                                                                               Penicillium solitum

              注: M1、M3~ M12、M14、M15、M17~ M25 为分离菌株编号ꎮ 下同ꎮ
              Note: M1ꎬ M3-M12ꎬ M14ꎬ M15ꎬ M17-M25 are the numbers of isolated strains. The same below.


            分离出了毛霉、根霉和青霉等腐生真菌ꎬ这些真菌                             种多糖降解酶类物质( Lange et al.ꎬ2019)ꎬ从而影
            不能引起健康魔芋球茎发病ꎬ它们在软腐病发病                              响魔芋球茎健康部位ꎬ加快软腐病的发病速度ꎮ
            过程中具体有何作用ꎬ还有待进一步研究ꎮ 但是ꎬ                            此外ꎬ我们还从魔芋软腐病状组织病健交界处分
            这些腐生真菌可以加速软腐组织的降解(Kavkler &                        离出了粉红螺旋聚孢霉属真菌ꎬ它是一种生防菌ꎬ
            Demšarꎬ2019)ꎬ在其快速繁殖的过程也会分泌各                        能够 捕 食 真 菌 和 线 虫 ( Seenivasagan & Babalolaꎬ
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