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Guihaia Feb. 2024ꎬ 44(2): 281-290
DOI: 10.11931/ guihaia.gxzw202211036
魏丽琴ꎬ 种培芳ꎬ 包新光ꎬ 等ꎬ 2024. 三个玫瑰品种花色物质的代谢组学分析 [J]. 广西植物ꎬ 44(2): 281-290.
WEI LQꎬ CHONG PFꎬ BAO XGꎬ et al.ꎬ 2024. Metabolomics analysis of flower color substances in three Rosa rugosa cultivars
[J]. Guihaiaꎬ 44(2): 281-290.
三个玫瑰品种花色物质的代谢组学分析
魏丽琴ꎬ 种培芳 ꎬ 包新光ꎬ 何海玲ꎬ 李清清
∗
( 甘肃农业大学 林学院ꎬ 兰州 730070 )
摘 要: 玫瑰(Rosa rugosa)具有很高的观赏价值和商业价值ꎬ但其花色比较单一ꎬ限制了玫瑰的开发利用及
其在园林造景中的应用ꎮ 为了探究‘苦水玫瑰’‘墨红玫瑰’和‘保加利亚白玫瑰’3 个不同品种玫瑰的呈色
物质ꎬ该研究利用超高效液相色谱-四级杆-飞行时间质谱(UPLC ̄Q ̄TOF ̄MS) 联用检测花瓣类黄酮的种类
和含量ꎬ通过 KEGG 数据库对差异代谢物进行富集分析ꎬ筛选出关键代谢物ꎬ并分析与花色表型值的相关
性ꎮ 结果表明:(1)在 3 个不同色系玫瑰花瓣中共检测到 58 种代谢物ꎬ其中花青素只有一种为矢车菊 ̄3 ̄O
葡萄糖苷ꎬ约占 30.45%ꎮ (2)K ̄means 聚类分析表明ꎬ共有 12 种关键代谢物注释到 KEGG 代谢通路中ꎬ其中
乔松素和杨梅黄酮是决定‘苦水玫瑰’和‘墨红玫瑰’花色呈红色的主要物质ꎬ圣草酚、木犀草素和山萘酚是
决定‘保加利亚白玫瑰’花色呈白色的主要物质ꎮ 该研究结果可为具有特定颜色玫瑰的育种提供理论依据ꎬ
并促进玫瑰在园林绿化中的应用ꎮ
关键词: 玫瑰品种ꎬ 花色物质ꎬ 代谢组学ꎬ UPLC ̄Q ̄TOF ̄MSꎬ 相关性
中图分类号: Q946 文献标识码: A 文章编号: 1000 ̄3142(2024)02 ̄0281 ̄10
Metabolomics analysis of flower color substances
in three Rosa rugosa cultivars
∗
WEI Liqinꎬ CHONG Peifang ꎬ BAO Xinguangꎬ HE Hailingꎬ LI Qingqing
( College of Forestryꎬ Gansu Agricultural Universityꎬ Lanzhou 730070ꎬ China )
Abstract: Rosa rugosa is a deciduous shrub belonging to Rosa L. in Rosaceae. It has a high ornamental value and
commercial valueꎬ but its single color limits the development and utilization of rose and its application in landscape
architecture. In order to explore the coloring substances of three different varieties of rosesꎬ ‘ Rosa rugosa × Rosa
sertata’ꎬ ‘Rosa Crimson Glory’ and ‘ Rosa alba’ꎬ this study used ultra ̄high performance liquid chromatography ̄
quadrupole ̄time ̄of ̄flight mass spectrometry ( UPLC ̄Q ̄TOF ̄MS) to detect the types and contents of flavonoids in
petals. The KEGG database was used to enrich the differential metabolitesꎬ screen out the key metabolitesꎬ and analyze
the correlation with the phenotypic value of flower color. The results were as follows: (1) A total of 58 metabolites were
detected in the petals of three different color rose varietiesꎬ of which only one anthocyanin was cyanidin ̄3 ̄O ̄glucosideꎬ
收稿日期: 2023-06-02
基金项目: 甘肃省自然科学基金(20JR5RA035)ꎮ
第一作者: 魏丽琴(1996-)ꎬ硕士研究生ꎬ研究方向为园林植物与应用ꎬ(E ̄mail)2570766505@ qq.comꎮ
∗
通信作者: 种培芳ꎬ博士ꎬ教授ꎬ研究方向为园林植物及荒漠植物生理生态ꎬ(E ̄mail)zhongpf@ gsau.edu.cnꎮ