Page 132 - 《广西植物》2026年第3期
P. 132

5 0 8                                  广  西  植  物                                         46 卷
                                         1                1                  1               1
                          LONG Yanyan ꎬ HUANG Ziyi ꎬ ZENG Fenghua ꎬ ZHANG Yan ꎬ
                                                 2               1            1∗
                                         XIE Fei ꎬ ZHAO Huili ꎬ XIE Ling

             ( 1. Institute of Plant Protection / Key Laboratory of South China Fruit and Vegetable Green Controlꎬ Ministry of Agriculture and Rural Affairs /
                    Guangxi Key Laboratory of Biology of Crop Diseases and Pestsꎬ Guangxi Academy of Agricultural Sciencesꎬ Nanning 530007ꎬ
                               Chinaꎻ 2. Guangxi Huace Testing and Certification Co.ꎬ Ltd.ꎬ Nanning 530007ꎬ China )

                 Abstract: Curcuma kwangsiensis is a Daodi medicinal material of Curcuma in Zingiberaceaeꎬ yet the mechanism underlying
                 its quality formation remains unclear. To elucidate the interaction relationships between endophytic fungal communitiesꎬ soil
                 environmentsꎬ and medicinal material qualityꎬ and to reveal the microbiological basis of its geographical authenticity.
                 C. kwangsiensis samples were collected from three major production areas in Qinzhou City (Pubei Countyꎬ Lingshan
                 Countyꎬ and Qinnan District). Using ITS high ̄throughput sequencing and chemical analysis methodsꎬ the endophytic fungal
                 community structures in five tissues ( rootꎬ rhizomeꎬ stemꎬ leafꎬ and flower) were systematically studiedꎬ and the
                 physicochemical properties of the rhizosphere soil and quality markers of the medicinal materials were analyzed. Redundancy
                 analysis (RDA) and correlation analysis were employed to explore the relationships between microbial communitiesꎬ
                 environmental factorsꎬ and medicinal material quality. The results were as follows: (1) A total of 2 357 operational
                 taxonomic units (OTUs) (902 species of fungi) were obtained from 45 samplesꎻ the distribution of OTUs among the three
                 regions differed significantlyꎬ with only 19.4% being common core OTUs across regionsꎻ leaf tissues had the highest number
                 of total OTUs (1 205) and unique OTUs (415)ꎬ while the shared OTUs among the five tissue types accounted for only
                 6.9%. (2) Above ̄ground tissues (leaf and flower) exhibited significantly higher fungal richness (ACE and Chao 1 indexes)
                 and diversity (Shannon index) than below ̄ground tissues (root and rhizome). Additionallyꎬ NMDS analysis revealed
                 distinct differences in community structures between above ̄ground and below ̄ground tissues. (3) The core genus analysis
                 indicated that the below ̄ground tissues were dominated by taxa such as unclassified - c — Sordariomycetes and wood ̄
                 decomposing fungiꎬ while the above ̄ground tissues were characterized by dominant genera like Cladosporium and
                 Alternaria. The stem tissues displayed transitional characteristics in their fungal composition. (4) The RDA demonstrated
                 that soil pHꎬ organic matterꎬ and nutrients (nitrogenꎬ phosphorusꎬ and potassium) significantly influenced the fungal
                 community compositionꎻ rhizome samples from Pubei County showed the highest olibanum alcohol ̄soluble extract content
                 (ASEC). Notablyꎬ the rhizome fungal community was closely associated with ASEC levelsꎬ and unclassified - c —
                 Sordariomycetes in rhizomes showed a positive correlation with ASECꎬ whereas Acremonium exhibited a negative correlation
                 with ASEC. The above results indicate that the endophytic fungal community in Curcuma kwangsiensis exhibits significant
                 regional specificity and tissue niche differentiation characteristic. Specific fungal taxa may participate in the accumulation of
                 medicinal compounds through microenvironmental regulationꎬ providing new insights into the microbial mechanisms
                 underlying the formation of Daodi medicinal material quality.
                 Key words: Curcuma kwangsiensisꎬ endophytic fungiꎬ high ̄throughput sequencingꎬ tissue specificityꎬ soil
                 physicochemical properties




                  广 西 莪 术 ( Curcuma kwangsiensis) 为 姜 科        完全阐明ꎮ 孙思胜等(2024) 研究发现ꎬ内生真菌
            (Zingiberaceae)姜黄属( Curcuma) 道地药材ꎬ主产               或通过代谢调控途径参与药材道地性形成ꎬ这一
            于广西钦州、玉林等地ꎬ其根茎( 莪术) 和块根( 郁                         发现为解析莪术品质形成机制提供了新视角ꎮ
            金)富含莪术醇、莪术二酮等活性成分ꎬ具有显著                                 植物内生真菌是指生活史的一定阶段或全部
            的抗 肿 瘤 和 抗 炎 药 理 活 性 ( 朱 善 岚 和 黄 品 芳ꎬ              阶段 生 活 于 植 物 组 织 内 部 的 真 菌 ( He et al.ꎬ
            2007ꎻ张贵杰和黄克斌ꎬ2015ꎻ刘布 鸣 等ꎬ2023)ꎮ                    2023)ꎮ 作为植物微生态系统的核心成员ꎬ内生真
            作为 “ 桂 十 味” 代 表 性 品 种 ( 邓 佳 刚 和 韦 松 基ꎬ             菌与宿主长期协同进化ꎬ表现出促进植物生长( 陈
            2007ꎻ刘布鸣等ꎬ2023)ꎬ其药材品质形成机制尚未                        建桦等ꎬ2019ꎻ刘圣越等ꎬ2021ꎻ罗琳等ꎬ2021)、增
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