Page 43 - 《广西植物》2026年第6期
P. 43

9 5 8                                  广  西  植  物                                         46 卷
               ( 1. College of Pharmacyꎬ Guangxi University of Chinese Medicineꎬ Nanning 530200ꎬ Chinaꎻ 2. University Engineering Research Center of
              Characteristic Traditional Chinese Medicine and Ethnomedicineꎬ Guangxi University of Chinese Medicineꎬ Nanning 530200ꎬ Chinaꎻ 3. Guangxi
               Key Laboratory of Zhuang and Yao Ethnic Medicineꎬ Guangxi University of Chinese Medicineꎬ Nanning 530200ꎬ Chinaꎻ 4. Guangxi Zhuang
                      Autonomous Region Engineering Research Centre for High ̄Value Utilisation of Gui ̄Produced Local Medicinal Materialsꎬ
                        Nanning 530200ꎬ Chinaꎻ 5. Guangxi Guiping Yueda Perfume Corporationꎬ Guigang 537200ꎬ Guangxiꎬ China )

                 Abstract: To investigate the effects of the endophytic fungus RG170 ( Subsessila turbinata) on the physiological
                 characteristics and secondary metabolite accumulation of Cinnamomum cassia seedlingsꎬ the endophytic fungus RG170ꎬ
                 isolated from healthy leaves of C. cassiaꎬ was used as the research subject. The plant growth ̄promoting potential was
                 evaluated through in vitro assays assessing phosphate solubilizationꎬ potassium solubilizationꎬ and nitrogen fixationꎬ as
                 well as through a sterile co ̄culture system. Pot experiments were further conducted to verify its actual effects on C. cassia
                 growth and metabolism. The results were as follows: (1) Under sterile co ̄culture conditionsꎬ inoculation with RG170
                 significantly promoted the growth of C. cassia seedlings. Compared with the controlꎬ plant heightꎬ fresh weightꎬ number
                 of lateral rootsꎬ and leaf area increased by 15.4%ꎬ 54.2%ꎬ 40.1%ꎬ and 29.5%ꎬ respectivelyꎬ with all parameters except
                 plant height showing significant differences. (2) In pot experimentsꎬ inoculation with RG170 significantly increased the
                 dry biomass of the underground parts of C. cassia seedlings. (3) Compared with the controlꎬ the RG170 ̄inoculated
                 seedlings exhibited significantly higher levels of soluble sugars and soluble proteins in leavesꎬ with increases of 51.9%
                 and 63.8%ꎬ respectivelyꎬ while the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly
                 enhanced by 66.5% and 279.0%ꎬ respectively. (4) Inoculation also significantly increased total flavonoid and total
                 phenolic contents in leaves by 115.3% and 113.9%ꎬ respectivelyꎻ the contents of cinnamyl alcoholꎬ cinnamic acidꎬ and
                 cinnamaldehyde in both leaves and stem bark were significantly elevatedꎬ with cinnamaldehyde content increasing by
                 35.5% and 68.8%ꎬ respectively. In conclusionꎬ inoculation with the RG170 strainꎬ which possesses multiple growth ̄
                 promoting traitsꎬ not only promotes the growth and enhances the physiological activity of C. cassia seedlingsꎬ but also
                 facilitates the accumulation of key secondary metabolitesꎬ such as total flavonoids and cinnamaldehyde. This study
                 provides a basis for the development of microbial inoculants for C. cassia.
                 Key words: Cinnamomum cassiaꎬ endophytic fungiꎬ growth ̄promoting propertiesꎬ physiological characteristicsꎬ
                 cinnamaldehydeꎬ microbial agents



                肉桂(Cinnamomum cassia) 是樟科樟属多年生                     中药材的产量与品质受 诸 多 环 境 因 素 的 影
            乔木ꎬ主要种植于亚热带地区ꎬ在我国两广地区及                             响ꎬ这些因素包括非生物性因素与生物性因素ꎮ
            云南等地大面积种植ꎬ是广西重点发展的“ 桂十                             在以往的研究中ꎬ非生物性因素如光照( 李冠男
            味”药材之首( 陈晓璐等ꎬ2021)ꎬ其干燥树皮及枝                         等ꎬ2023)、温度( 李彩红等ꎬ2023)、水分( 金昆等ꎬ

            均可作中药材ꎬ并且作为香料在国际上享有盛誉ꎮ                             2025)等受到人们重视ꎬ而生物性因素时常被忽
            肉桂含有挥发油、多糖、倍半萜、黄酮类等多种成                             略ꎮ Liu 等(2021)研究发现ꎬ生物性因素中的内生
            分(高铭哲等ꎬ2021)ꎬ其中挥发油为其主要药用成                          真菌对药用植物的生长发育与次生代谢具有重要
            分(国家药典委员会ꎬ2020)ꎬ而肉桂醛是挥发油的                          作用ꎮ 内生真菌是存在于健康植物体内并能和宿

            核心活性物质(刘婕琳等ꎬ2024)ꎬ具有抗炎、抗菌、                         主共生的一类真菌ꎮ 有研究表明ꎬ内生真菌可促
            抗氧化及扩张血管等作用( 王清泉等ꎬ2024)ꎮ 作                         进药用植物生长( Khalil et al.ꎬ2021ꎻKhalmuratova
            为全球重要的香料和药材ꎬ肉桂市场需求持续增                              et al.ꎬ2021)、生物活性成分积累( Lindblom et al.ꎬ
            长(李桂兰等ꎬ2025)ꎮ 然而ꎬ不同产地的肉桂品质                         2018ꎻMastan et al.ꎬ2019) 以及新型活性化合物生
            良莠不齐(朱振玲等ꎬ2019)ꎬ部分地区的肉桂中肉                          成(Singh et al.ꎬ2022)ꎬ从而影响药用植物的品质ꎮ
            桂醛含量普遍低于药典标准ꎬ不仅制约了肉桂产                              白洋等(2022)发现ꎬ接种内生真菌能够显著增加
            业的健康发展ꎬ也凸显了从栽培源头提升肉桂品                              朝鲜淫羊藿的生物量ꎬ提高朝鲜淫羊藿总黄酮醇
            质的迫切性ꎮ                                             苷及总黄酮含量ꎻ吴慧等(2024) 发现ꎬ菌株 NS1、
   38   39   40   41   42   43   44   45   46   47   48