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4 期             刘夔等: 基于 EST ̄SSR 的钟花樱与近缘种的聚类分析及嫁接亲和性研究                                       7 6 5

                                          表 2  17 个多态性 EST SSRs 的引物特征
                                       Table 2  Characteristics of 17 polymorphic EST ̄SSRs
             引物名称
                        来源         重复类型       引物序列                        SSR 位置  E 值           参考文献
              Name of
                       Origin      Repeat type  Primer sequence          SSR location  E value  Reference
              primer
                                                                                  Hypothetical protein
                    Prunus jamasakura         F: ATGATGCTACCACAAGGGACTCGT
             AM287842            (TC) 4 GC(TC) 4                          Non ̄CDS  [Vitis vinifera]  Yoshiaki et al.ꎬ 2009
                      EST ̄SSRs                R: GTTTAGCTGCACATACGCTTTTACCTCC         ̄46
                                                                                  (2×10 )
                                                                                  Hypothetical protein
                    Prunus jamasakura         F: ACACTCGGATCATAGAAATCTCCG
             DY638687               (TCT) 4                               Non ̄CDS  [Vitis vinifera]  Yoshiaki et al.ꎬ 2009
                      EST ̄SSRs                R: GTTTCCTGAAACCCTGTTGTCGAAT            ̄36
                                                                                  (3×10 )
                                                                                  Hypothetical protein
                    Prunus jamasakura         F: ACAACTCTTTCTGGGTTCATTGCT
             DY640364               (GGT) 4                                 CDS   [Vitis vinifera]  Yoshiaki et al.ꎬ 2009
                      EST ̄SSRs                R: GTTTAAAACTCGTATGCTTCCCAAGGGT         ̄42
                                                                                  (1×10 )
                                                                                  Unnamed
                    Prunus jamasakura         F: ACACTCGGATCATAGAAATCTCCG         protein product
             DY640849               (AG) 19                               Non ̄CDS               Yoshiaki et al.ꎬ 2009
                      EST ̄SSRs                R: GTTTCCTGAAACCCTGTTGTCGAAT        [Vitis vinifera]
                                                                                      ̄56
                                                                                  (3×10 )
                                                                                  Unknown protein
                    Prunus jamasakura         F: ATGATGCTACCACAAGGGACTCGT
             AM287648               (GAA) 5                                 CDS   [Arabidopsis thaliana]  Yoshiaki et al.ꎬ 2009
                      EST ̄SSRs                R: GTTTAGCTGCACATACGCTTTTACCTCC         ̄51
                                                                                  (6×10 )
                    Prunus jamasakura         F: ACTGACTCATGCGGTTATATGCAG
             DN553427            (TC) 3 TG(TC) 4                          Non ̄CDS  —            Yoshiaki et al.ꎬ 2009
                      EST ̄SSRs                R: GTTTAAGGTCGACAACTACTGTACAATGC
                    Prunus jamasakura         F: ATAGTGCAGTTGAGAAACGAGCAG
             DN554499             (TG) 6 (AG) 10                          Non ̄CDS  —            Yoshiaki et al.ꎬ 2009
                      EST ̄SSRs                R: GTTTAAGGTGCAGTTCGTTGTCGATGT
                    Prunus jamasakura (TA) 3 ATCCCCTCCCACC F: AGACACCCTATCACAATGTCGCAA
             DY647422                                                     Non ̄CDS  —            Yoshiaki et al.ꎬ 2009
                      EST ̄SSRs  ACGAAAATATCC(TC) 5  R: GTTTGCGAACAGCGATAACCTTAATCC
                                                                                  DNA ̄binding protein
                    Prunus jamasakura         F: ACCTCGCTTTTAAGTCTCCCTTGA         NtWRKY3
             DY650749               (TC) 8                                Non ̄CDS               Yoshiaki et al.ꎬ 2009
                      EST ̄SSRs                R: GTTTGAGACGGTGAAGTCGGTGATTTCT     [Nicotiana tabacum]
                                                                                      ̄26
                                                                                  (1×10 )
                                                                                  Predicted:
                      GenBank:                                                    transcription factor  自己设计合成
                                              F: CACACACACCTTCTCTCTCCTG
               PC1    EE489070.1   (TTTTA) 3                              Non ̄CDS  divaricata  [ Prunus  Design and synthesis
                                              R: GTTGTTATTGGTCTTGCTGCTG
                      EST ̄SSRs                                                    mume]         by myself
                                                                                      ̄11
                                                                                  (4×10 )
                                                                                  Hypothetical  protein
                      GenBank:                                                                  自己设计合成
                                              F: CAGCCGAACCCTAACAACTCTA           PRUPE_ppa016199mg
               PC3    EE488462.1    (TC) 16                               Non ̄CDS               Design and synthesis
                                              R: GAGTACCCTGTTGATCCTGAGC           [Prunus persica]
                      EST ̄SSRs                                                        ̄82        by myself
                                                                                  (4×10 )
                                                                                  Hypothetical  protein
                      GenBank:                                                                  自己设计合成
                                              F: AAGGAGAAAGGGAGCTACAACC           PRUPE_ppa011869mg
               PC6    EE488045.1    (GCA) 6                                 CDS                 Design and synthesis
                                              R: AGCCAAGGAGACAACAGAGAAG           [Prunus persica]
                      EST ̄SSRs                                                        ̄71        by myself
                                                                                  (1×10 )
                      GenBank:                                                                  自己设计合成
                                              F: CATGGTGTGGAGCAAAGATTTA
               PC8    EE488711.1   (AAATC) 5                              Non ̄CDS  —            Design and synthesis
                                              R: GTTGATACAGATAGGAAGCGGG
                      EST ̄SSRs                                                                  by myself
                                                                                  Hypothetical  protein
                      GenBank:                                                                  自己设计合成
                                              F: GGCACAAAAGAGAGGAACTTGT           PRUPE_ppa002937mg
               PC10   EE488225.1    (GCT) 6                                 CDS                 Design and synthesis
                                              R: AGGGTTACAGCCTCAATACCAA           [Prunus persica]
                      EST ̄SSRs                                                        ̄112       by myself
                                                                                  (4×10  )
                                                                                  Hypothetical  protein
                      GenBank:                                                                  自己设计合成
                                              F: CCAAGCTGACCTAACTTGACCT           PRUPE_ppa006091mg
               PC11   EE488161.1    (TTC) 10                                CDS                 Design and synthesis
                                              R: TAGCCCCACATGCTACCTCTAT           [Prunus persica]
                      EST ̄SSRs                                                                  by myself
                                                                                  (2×10  ̄113  )
                                                                                  Hypothetical  protein
                      GenBank:                                                                  自己设计合成
                                              F: ATATGGGCTGCGTTTATATTGG           PRUPE_ppa002580mg
               PC12   DV175423.1   (AGA) 12                               Non ̄CDS               Design and synthesis
                                              R: GATTGCACATGCCTTTGTCTTA           [Prunus persica]
                      EST ̄SSRs                                                        ̄31        by myself
                                                                                  (2×10 )
                                                                                  Predicted:
                      GenBank:                                                    photosystem Ⅱ 5 kDa  自己设计合成
                                              F: AATTTGCAGAGATGGCTTCC
               PC17   EE487895.1    (AGC) 7                                 CDS   proteinꎬ chloroplastic  Design and synthesis
                                              R: CTTCTCCTTGGCTTCTTTTGTC
                      EST ̄SSRs                                                    [Prunus mume]  by myself
                                                                                      ̄34
                                                                                  (3×10 )
              注: CDS. 编码区域ꎻ Non ̄CDS. 非编码区域ꎮ — 表示未发现ꎮ
              Note: CDS. Coding regionꎻ Non ̄CDS. Non ̄coding region. — indicates not discovered.
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