Page 85 - 《广西植物》2024年第7期
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7 期 曾婉俐等: 烟草香气相关基因 CRISPR / Cas9 编辑突变体库的构建 1 2 8 5
续表 1
编辑率 脱靶基因 编辑率
序号 靶位点序列 靶基因编号 基因功能注释 编辑 编辑
PAM Editing Off ̄target Editing
No. Target site sequence Target gene No. Gene annotation Editing Editing
rate gene rate
41 TCCGAGGATCACCACCCAAT GGG XM_016640747.1 Sterol 3 ̄beta ̄ 是 7 / 7
glucosyltransferase Yes
42 AAATGTCCTAAGGAAAGCTG AGG XM_016595403.1 Sucrose synthase 是 7 / 7
Yes
43 GAGGAGGGCTATTGCTGGAA CGG NM_001325834.1 Beta ̄carotene 是 7 / 7
hydroxylase 2 Yes
XM_009782556.1 Beta ̄carotene 是 3 / 7
hydroxylase 2 Yes
44 GGAAGCGAATCTACGTAGGC AGG XR_001644690.1 Soluble starch 是 6 / 7
synthase 3 Yes
45 ATGGAGTTGAATTCCTCAAT AGG XM_016618980.1 Sucrose synthase 是 7 / 7
Yes
XM_016604255.1 Sucrose synthase 是 5 / 7
Yes
46 TACAAATGTGGAAAAGCCGG TGG XM_016646488.1 Sterol 3 ̄beta ̄ 是 6 / 7
glucosyltransferase Yes
47 ATACTGGGAGCAACTGAGCT GGG XM_016630467.1 Cysteine ̄rich 是 7 / 7
receptor like Yes
48 GTTGTGGGGCATGAGATTAC TGG XM_016650499.1 Alcohol 是 7 / 7 XM_016656110.1 否 0 / 7
dehydrogenase Yes No
49 TGGGCAAAGGAGGAGCAAAG AGG XM_016645712.1 Histone 是 7 / 7
Yes
50 AGCTTCTTTGGCAACAGACT TGG XM_016624910.1 Peroxidase 是 7 / 7 XM_016635038.1 否 0 / 7
Yes No
51 ATATCATCACGAATATATCG CGG XM_016612129.1 2 ̄oxoglutarate 是 4 / 7
(2OG) and Yes
Fe(II) ̄dependent
oxygenase
52 TGGGATTGAGCTGTGGGTAT TGG XM_016609474.1 Pectate lyase 是 7 / 7
Yes
53 GCTGTCGAAAATGCATGCGG TGG XM_016614797.1 Peroxidase N ̄like 是 7 / 7 XM_016623966.1 否 0 / 7
Yes No
54 TCGACTATAAGTGTTCCTTC AGG XM_016650365.1 Ferredoxin 是 7 / 7
Yes
55 ATTGCCTTAGGTTACGAAGC GGG XM_016635292.1 Dehydrodolichyl 是 7 / 7
diphosphate synthase Yes
55 XM_016628980.1 Dehydrodolichyl 是 7 / 7
diphosphate synthase Yes
56 ATATGGTTCACCAAACCAAT GGG XM_016616473.1 Alpha carbonic 是 7 / 7
anhydrase Yes
57 GCAGGGTGAGCCGACTCAAC AGG XM_016606799.1 V ̄type proton 是 7 / 7
ATPase Yes
58 AACCTCTGTATGAGGGCACG AGG XM_016594514.1 Oxygne ̄evolving 是 7 / 7 XM_016609317.1 否 0 / 7
enhancer Yes No
59 ATAAGCAGTCAACCCCACAG CGG XM_016622845.1 DNA replication 是 7 / 7
licensing factor Yes
60 AAGTGGGATCCCAACCTCTT TGG XM_016634593.1 Deoxyloganetic 是 7 / 7
acid glucosyl ̄ Yes
transferase