Page 85 - 《广西植物》2024年第7期
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7 期                曾婉俐等: 烟草香气相关基因 CRISPR / Cas9 编辑突变体库的构建                                 1 2 8 5

                                                          续表 1
                                                                                编辑率    脱靶基因            编辑率
             序号         靶位点序列                   靶基因编号       基因功能注释         编辑                    编辑
                                         PAM                                    Editing  Off ̄target    Editing
             No.      Target site sequence     Target gene No.  Gene annotation  Editing         Editing
                                                                                 rate    gene           rate
              41  TCCGAGGATCACCACCCAAT   GGG  XM_016640747.1  Sterol 3 ̄beta ̄  是  7 / 7
                                                            glucosyltransferase  Yes
              42  AAATGTCCTAAGGAAAGCTG   AGG  XM_016595403.1  Sucrose synthase  是  7 / 7
                                                                           Yes
              43  GAGGAGGGCTATTGCTGGAA   CGG  NM_001325834.1  Beta ̄carotene  是   7 / 7
                                                            hydroxylase 2  Yes
                                              XM_009782556.1  Beta ̄carotene  是   3 / 7
                                                            hydroxylase 2  Yes
              44  GGAAGCGAATCTACGTAGGC   AGG   XR_001644690.1  Soluble starch  是  6 / 7
                                                            synthase 3     Yes

              45  ATGGAGTTGAATTCCTCAAT   AGG  XM_016618980.1  Sucrose synthase  是  7 / 7
                                                                           Yes
                                              XM_016604255.1  Sucrose synthase  是  5 / 7
                                                                           Yes
              46  TACAAATGTGGAAAAGCCGG   TGG  XM_016646488.1  Sterol 3 ̄beta ̄  是  6 / 7
                                                            glucosyltransferase  Yes
              47  ATACTGGGAGCAACTGAGCT   GGG  XM_016630467.1  Cysteine ̄rich  是   7 / 7
                                                            receptor like  Yes
              48  GTTGTGGGGCATGAGATTAC   TGG  XM_016650499.1  Alcohol       是    7 / 7 XM_016656110.1  否  0 / 7
                                                            dehydrogenase  Yes                    No
              49  TGGGCAAAGGAGGAGCAAAG   AGG  XM_016645712.1  Histone       是    7 / 7
                                                                           Yes
              50  AGCTTCTTTGGCAACAGACT   TGG  XM_016624910.1  Peroxidase    是    7 / 7 XM_016635038.1  否  0 / 7
                                                                           Yes                    No
              51  ATATCATCACGAATATATCG   CGG  XM_016612129.1  2 ̄oxoglutarate  是  4 / 7
                                                            (2OG) and      Yes
                                                            Fe(II) ̄dependent
                                                            oxygenase
              52  TGGGATTGAGCTGTGGGTAT   TGG  XM_016609474.1  Pectate lyase  是   7 / 7
                                                                           Yes
              53  GCTGTCGAAAATGCATGCGG   TGG  XM_016614797.1  Peroxidase N ̄like  是  7 / 7 XM_016623966.1  否  0 / 7
                                                                           Yes                    No
              54   TCGACTATAAGTGTTCCTTC  AGG  XM_016650365.1  Ferredoxin    是    7 / 7
                                                                           Yes
              55  ATTGCCTTAGGTTACGAAGC   GGG  XM_016635292.1  Dehydrodolichyl  是  7 / 7
                                                            diphosphate synthase Yes

              55                              XM_016628980.1  Dehydrodolichyl  是  7 / 7
                                                            diphosphate synthase Yes
              56  ATATGGTTCACCAAACCAAT   GGG  XM_016616473.1  Alpha carbonic  是  7 / 7
                                                            anhydrase      Yes
              57  GCAGGGTGAGCCGACTCAAC   AGG  XM_016606799.1  V ̄type proton  是   7 / 7
                                                            ATPase         Yes
              58  AACCTCTGTATGAGGGCACG   AGG  XM_016594514.1  Oxygne ̄evolving  是  7 / 7 XM_016609317.1  否  0 / 7
                                                            enhancer       Yes                    No

              59  ATAAGCAGTCAACCCCACAG   CGG  XM_016622845.1  DNA replication  是  7 / 7
                                                            licensing factor  Yes
              60  AAGTGGGATCCCAACCTCTT   TGG  XM_016634593.1  Deoxyloganetic  是  7 / 7
                                                            acid glucosyl ̄  Yes
                                                            transferase
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