Page 84 - 《广西植物》2024年第7期
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1 2 8 4                                广  西  植  物                                         44 卷
                                                          续表 1
                                                                                编辑率    脱靶基因            编辑率
             序号         靶位点序列                   靶基因编号       基因功能注释         编辑                    编辑
                                         PAM                                    Editing  Off ̄target    Editing
             No.      Target site sequence     Target gene No.  Gene annotation  Editing         Editing
                                                                                 rate    gene           rate
              20  GGAGACGGCTTGTACAACGG   CGG  XM_016616459.1  Abscisic acid  是   7 / 7
                                                            receptor PYL   Yes
              21  TGTCCAAGAGACCGTCGGGT   AGG  NM_001325338.1  Chlorophyll a ̄b  否  0 / 7
                                                            binding protein  No
                                              XM_016644018.1  Chlorophyll a ̄b  否  0 / 7
                                                            binding protein  No
              22  CTCCTCTGCAAGACGGCCAC   GGG  XM_016635355.1  SUMO ̄conjugating  是  7 / 7
                                                            enzyme         Yes
                                              XM_016620991.1  SUMO ̄conjugating  是  7 / 7
                                                            enzyme         Yes
              23  CTATGATAAGTCTGTACCAG   AGG  XM_016637410.1  Sucrose synthease  是  7 / 7 XM_016579318.1  否  0 / 7
                                                                           Yes                    No
              24  GCTGCGGTTGCATGGGAAGC   CGG  XM_016647041.1  Alcohol       是    7 / 7
                                                            dehydrogenase  Yes
              25  CTGGAGAGTTGCCTAGTGGT   TGG  XM_016602081.1  Amidase       是    6 / 7 XM_016629727.1  否  0 / 7
                                                                           Yes                    No
              26  TTGGTTCGAGGAAGTTGCGG   AGG  XM_016645977.1  Jasmonic acid ̄  否  0 / 7 XM_016580920.1  否  0 / 7
                                                            amido synthetae  No                   No
              27  AGAAGGCAGAGTAAAGACAG   AGG  XM_016642986.1  Quinolinate   是    7 / 7
                                                            synthase       Yes
              28  GAGACCTGCAGATCTACCCG   CGG  NM_001325524.1  Reticuline oxidase  是  7 / 7 NM_001326242.1  否  0 / 7
                                                                           Yes                    No
              29  CAAGCTGTAGGTTCCCGAGT   TGG  NM_001325814.1  Detoxification  是  6 / 7 NM_001325543.1  否  0 / 7
                                                                           Yes                    No
              30  ATATCCATCCATTCAAGCGA   TGG  NM_016622703.1  Cytochrome    是    7 / 7
                                                            P450 82C4      Yes
              31  ATGCGGATGAGGCTCGTCGT   AGG  XM_016658217.1  Importin subunit  是  7 / 7
                                                                           Yes
              32  TTTGCAAGGGAGACGTCATT   TGG  NM_001325509.1  MLO ̄like protein  是  5 / 7 XM_016633095.1  否  0 / 7
                                                                           Yes                    No
              33  AGCGTTCTGTGTTGCAGACA   AGG  XM_016647590.1  Reticuline oxidase  否  0 / 7
                                                                            No
              34  TTGCCCGTAATCCAAAAGGC   TGG  NM_001325865.1  Caffeoyl ̄CoA  是    7 / 7
                                                            O ̄methltransferase  Yes
              35  TCTGTTGTAACCGGCAAGAC   TGG  NM_001326113.1  Delta12 ̄fatty  是   5 / 7
                                                            acid dehydrogenase  Yes
                                              XM_016659636.1  Delta12 ̄fatty  是   5 / 7
                                                            acid dehydrogenase  Yes
              36  TCGAAACAGACAAATAAACA   AGG  XM_016616941.1  ECERIFERUM    是    7 / 7
                                                                           Yes
              37  ATTGGTGACTGGGGACGTAA   AGG  XM_016597780.1  Purple acid   是    7 / 7
                                                            phosphatase    Yes
              38  GACCACCAGGGCACTGGCTC   TGG  NM_001324627.1  Endochitinase  是   7 / 7
                                                                           Yes
                                              XM_016653822.1  Endochitinase  是   6 / 7
                                                                           Yes
              39  GTTACCCGACCGGTCTTACA   AGG  XM_016599368.1  Phospholipase  是   2 / 7
                                                                           Yes
              40  CTTGGTCTGCCCGATACTGG   AGG  XM_016590747.1  Sucrose synthase  是  7 / 7 XM_016611295.1  否  0 / 7
                                                                           Yes                    No
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