Page 84 - 《广西植物》2024年第7期
P. 84
1 2 8 4 广 西 植 物 44 卷
续表 1
编辑率 脱靶基因 编辑率
序号 靶位点序列 靶基因编号 基因功能注释 编辑 编辑
PAM Editing Off ̄target Editing
No. Target site sequence Target gene No. Gene annotation Editing Editing
rate gene rate
20 GGAGACGGCTTGTACAACGG CGG XM_016616459.1 Abscisic acid 是 7 / 7
receptor PYL Yes
21 TGTCCAAGAGACCGTCGGGT AGG NM_001325338.1 Chlorophyll a ̄b 否 0 / 7
binding protein No
XM_016644018.1 Chlorophyll a ̄b 否 0 / 7
binding protein No
22 CTCCTCTGCAAGACGGCCAC GGG XM_016635355.1 SUMO ̄conjugating 是 7 / 7
enzyme Yes
XM_016620991.1 SUMO ̄conjugating 是 7 / 7
enzyme Yes
23 CTATGATAAGTCTGTACCAG AGG XM_016637410.1 Sucrose synthease 是 7 / 7 XM_016579318.1 否 0 / 7
Yes No
24 GCTGCGGTTGCATGGGAAGC CGG XM_016647041.1 Alcohol 是 7 / 7
dehydrogenase Yes
25 CTGGAGAGTTGCCTAGTGGT TGG XM_016602081.1 Amidase 是 6 / 7 XM_016629727.1 否 0 / 7
Yes No
26 TTGGTTCGAGGAAGTTGCGG AGG XM_016645977.1 Jasmonic acid ̄ 否 0 / 7 XM_016580920.1 否 0 / 7
amido synthetae No No
27 AGAAGGCAGAGTAAAGACAG AGG XM_016642986.1 Quinolinate 是 7 / 7
synthase Yes
28 GAGACCTGCAGATCTACCCG CGG NM_001325524.1 Reticuline oxidase 是 7 / 7 NM_001326242.1 否 0 / 7
Yes No
29 CAAGCTGTAGGTTCCCGAGT TGG NM_001325814.1 Detoxification 是 6 / 7 NM_001325543.1 否 0 / 7
Yes No
30 ATATCCATCCATTCAAGCGA TGG NM_016622703.1 Cytochrome 是 7 / 7
P450 82C4 Yes
31 ATGCGGATGAGGCTCGTCGT AGG XM_016658217.1 Importin subunit 是 7 / 7
Yes
32 TTTGCAAGGGAGACGTCATT TGG NM_001325509.1 MLO ̄like protein 是 5 / 7 XM_016633095.1 否 0 / 7
Yes No
33 AGCGTTCTGTGTTGCAGACA AGG XM_016647590.1 Reticuline oxidase 否 0 / 7
No
34 TTGCCCGTAATCCAAAAGGC TGG NM_001325865.1 Caffeoyl ̄CoA 是 7 / 7
O ̄methltransferase Yes
35 TCTGTTGTAACCGGCAAGAC TGG NM_001326113.1 Delta12 ̄fatty 是 5 / 7
acid dehydrogenase Yes
XM_016659636.1 Delta12 ̄fatty 是 5 / 7
acid dehydrogenase Yes
36 TCGAAACAGACAAATAAACA AGG XM_016616941.1 ECERIFERUM 是 7 / 7
Yes
37 ATTGGTGACTGGGGACGTAA AGG XM_016597780.1 Purple acid 是 7 / 7
phosphatase Yes
38 GACCACCAGGGCACTGGCTC TGG NM_001324627.1 Endochitinase 是 7 / 7
Yes
XM_016653822.1 Endochitinase 是 6 / 7
Yes
39 GTTACCCGACCGGTCTTACA AGG XM_016599368.1 Phospholipase 是 2 / 7
Yes
40 CTTGGTCTGCCCGATACTGG AGG XM_016590747.1 Sucrose synthase 是 7 / 7 XM_016611295.1 否 0 / 7
Yes No