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                          Guihaia  Feb. 2026ꎬ 46(2): 235-244

            DOI: 10.11931/ guihaia.gxzw202412042
            牛靓洁ꎬ 王文康ꎬ 丁慧莹ꎬ 等ꎬ 2026. 雪松花粉的淀粉粒在萌发过程中的变化 [J]. 广西植物ꎬ 46(2): 235-244.
            NIU L Jꎬ WANG W Kꎬ DING H Yꎬ et al.ꎬ 2026. Changes in starch granules in Cedrus deodara pollen during germination [J].
            Guihaiaꎬ 46(2): 235-244.


                          雪松花粉的淀粉粒在萌发过程中的变化


                                     牛靓洁 ꎬ 王文康 ꎬ 丁慧莹 ꎬ 王  伟                       1∗
                                                                      1
                                                          1
                                             1ꎬ2
                 ( 1. 河南农业大学 生命科学学院ꎬ 郑州 450046ꎻ 2. 信阳师范大学 茶学与食品学院ꎬ 大别山实验室ꎬ 河南 信阳 464000 )

                 摘  要: 裸子植物雪松(Cedrus deodara) 为风媒传粉植物ꎬ其花粉萌发和花粉管生长速率明显比被子植物
                 (如玉米)慢ꎮ 淀粉是风媒植物花粉萌发的主要能量来源ꎮ 为揭示雪松花粉淀粉粒的特征及其在花粉萌发
                 过程中的变化ꎬ该研究以雪松和玉米的花粉为材料ꎬ通过生理生化、细胞学和蛋白质组学手段比较花粉萌发
                 过程中淀粉粒的变化及其对花粉管生长的影响ꎮ 结果表明:(1)与玉米成熟花粉相比ꎬ雪松花粉中的淀粉含
                 量较低ꎬ但直链淀粉比例较高ꎮ (2)雪松花粉的淀粉粒呈不规则鹅卵石状ꎬ而玉米花粉的淀粉粒为短棒状ꎮ
                 (3)雪松花粉中的 α ̄淀粉酶和 β ̄淀粉酶的活性、同工酶种类和酶活性均低于玉米花粉ꎮ (4)雪松成熟花粉
                 中的淀粉粒主要分布在花粉壁内侧的细胞质中ꎬ在花粉萌发过程中逐渐进入花粉管ꎻ同时ꎬ淀粉同工酶种类
                 和酶活性增加ꎬ淀粉降解加强ꎬ可溶性糖含量增加ꎬ从而为花粉萌发提供能量ꎮ 该研究为阐明裸子植物风媒
                 花粉萌发和花粉管生长较慢的原因提供了新线索ꎮ
                 关键词: 风媒花粉ꎬ 雪松花粉ꎬ 淀粉粒ꎬ 花粉萌发ꎬ 淀粉酶活性ꎬ 淀粉结合蛋白

                 中图分类号: Q944.42    文献标识码: A    文章编号: 1000 ̄3142(2026)02 ̄0235 ̄10

                       Changes in starch granules in Cedrus deodara

                                       pollen during germination


                                      1ꎬ2                   1                  1              1∗
                        NIU Liangjie ꎬ WANG Wenkang ꎬ DING Huiying ꎬ WANG Wei

                     ( 1. College of Life Sciencesꎬ Henan Agricultural Universityꎬ Zhengzhou 450046ꎬ Chinaꎻ 2. Dabie Mountain Laboratoryꎬ
                            College of Tea and Food Scienceꎬ Xinyang Normal Universityꎬ Xinyang 464000ꎬ Henanꎬ China )
                 Abstract: The gymnosperm cedar(Cedrus deodara) is a wind ̄pollinated plantꎬ and exhibits a significantly slower process
                 of pollen germination and pollen tube growth than that of angiosperm (e.g.ꎬ maize). Starch is the main energy source for
                 pollen germination in wind ̄pollinated plants. To reveal the characteristics of starch granules in cedar pollen and their
                 changes during pollen germinationꎬ the pollens of cedar and maize were used as materials to compare the changes of starch
                 granules and their effects during pollen germination by physiological and biochemicalꎬ cytological and proteomic
                 approaches. The results were as follows: (1) Compared with mature maize pollenꎬ the starch content in cedar pollen was
                 lowerꎬ but the amylose proportion was higher. (2) Starch granules in cedar pollen were irregular and cobblestone ̄likeꎬ
                 whereas those in maize were short and rod ̄shaped. (3) The activities of α ̄amylase and β ̄amylaseꎬ and the types and


              收稿日期: 2025-05-25  接受日期: 2025-06-19
              基金项目: 国家自然科学基金联合基金(U1904107)ꎮ
              第一作者: 牛靓洁(1996—)ꎬ博士ꎬ讲师ꎬ研究方向为植物生长和逆境响应ꎬ(E ̄mail)niuliangjie@ xynu.edu.cnꎮ
            ∗
              通信作者: 王伟ꎬ博士ꎬ教授ꎬ研究方向为玉米耐旱的分子机制ꎬ(E ̄mail)wangwei@ henau.edu.cnꎮ
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